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Sample GSM4202151 Query DataSets for GSM4202151
Status Public on Dec 04, 2019
Title PRSS53-2
Sample type SRA
 
Source name rabbit skin
Organism Oryctolagus cuniculus
Characteristics tissue: skin
age: 30 days
genotype: Prss53-/-
Treatment protocol Crispr/Cas9
Growth protocol All rabbit experiments were conducted under the approval of the Animal Care Center and Use Committee of Jilin University. The rabbits were housed under standard laboratory conditions of a 12-h light and 12-h dark cycle in individual cages and were fed twice a day with commercial rabbit basic diet (Animal center of Jilin University) and water ad libitum.
Extracted molecule total RNA
Extraction protocol Skin were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent.
NEBNext Ultra RNA library Prep Kit for Illumina
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing Basecalls performed using Bcl2fastq (v2.17.1.14)
Filter Dataset using Cutadapt(version 1.9.1)
 
Submission date Dec 03, 2019
Last update date Dec 05, 2019
Contact name CNSA CNGBdb
E-mail(s) datasubs@cngb.org
Organization name CNGB
Street address Jinsha Road
City Shenzhen
State/province Guangdong
ZIP/Postal code 518000
Country China
 
Platform ID GPL26786
Series (1)
GSE141384 A direct link between Prss53, hair curvature, and skeletal dysplasia
Relations
BioSample SAMN13473201
SRA SRX7262700

Supplementary file Size Download File type/resource
GSM4202151_PRSS53-2.anno.fpkm.xls.gz 991.2 Kb (ftp)(http) XLS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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