|
| Status |
Public on Jul 13, 2009 |
| Title |
MDA-MB-436 |
| Sample type |
RNA |
| |
|
| Source name |
breast cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-436
cell type: breast cancer
morphology: spindle
|
| Growth protocol |
Cell lines were grown to optimal cell density in RPMI 1640 supplemented with 10% FBS at 37C. Medium was changed 24h prior to RNA isolation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were washed with PBS at 37C prior to isolation. Total RNA was isolated using the Rneasy kit from Qiagen. Lysis of the cells was performed in the culture flask directly after washing with PBS. Total RNA was DNAseI-treated for 1 hour at 37C. RNA QC using Agilent Technologies 2100 Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 10 ug total RNA.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Description |
Gene expression of MDA-MB-436.
|
| Data processing |
Intensity values for all genes were scaled using the global normalization factor as specified by GCOS 1.1.
|
| |
|
| Submission date |
Jun 24, 2009 |
| Last update date |
Jul 13, 2009 |
| Contact name |
Antoinette Hollestelle |
| E-mail(s) |
a.hollestelle@erasmusmc.nl
|
| Phone |
+31-10-7044372
|
| Fax |
+31-10-7044377
|
| Organization name |
Erasmus University Medical Center
|
| Department |
Medical Oncology
|
| Lab |
Genomics and Proteomics of Breast Cancer
|
| Street address |
Dr. Molenwaterplein 50
|
| City |
Rotterdam |
| ZIP/Postal code |
3015 GE |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE16795 |
Affymetrix HG-U133A gene expression profiles of 39 human breast cancer cell lines |
|
