|
Status |
Public on Sep 30, 2010 |
Title |
endometrium_progesterone+estrogen-treated_rep3 |
Sample type |
RNA |
|
|
Source name |
endometrial tissue, treated with progesterone+estrogen
|
Organism |
Bos taurus |
Characteristics |
tissue: endometrium agent: progesterone+estrogen
|
Treatment protocol |
Ovariectomized cows (n=12) were randomly divided into four groups; the control group (n=3) received saline treatment at Day 0 (beginning of the experiment), E2 groups (n=3) received saline and 1 mg estradiol benzoate (EB) at Day 0 and Day 5, respectively, two CIDR were inserted to the uterus of animals of the P4 group (n=3) from Day 0 to Day 6, and the E2+P4 group (n=3) received two CIDR at Day 0 and injection of 1 mg EB immediately after removal of the CIDR at Day 6. Blood samples (5 ml) in control and P4 groups were collected at Day 5 after saline or CIDR treatment, and in E2 and E2+P4 groups blood samples were collected after EB treatment. All animals were slaughtered 2 days after blood sampling, and endometrium samples were collected as described in Bauersachs et al J Mol Endocrinol 2005). The tissue samples were snap-frozen and stored at -80°C until analysis.
|
Growth protocol |
The cows (n=12) were ovariectomized, maintained in a free-stall barn throughout the experimental period, and had free access to food and water.
|
Extracted molecule |
total RNA |
Extraction protocol |
deep frozen tissue was transferred to 5 ml RNAlater-ICE (Ambion, Austin, TX, USA) and allowed to impregnate at -20°C during two days for optimal RNA protection. Total RNA was extracted from bovine mammary gland tissue with the Trizol method according to the manufacturers protocol
|
Label |
biotin
|
Label protocol |
total RNA was transcribed and labeled with Ambion Kit MessageAmp II Biotin Enhanced according to the manufacturers protocol
|
|
|
Hybridization protocol |
fragmented cRNA was hybridized to Affymetrix GeneChip Bovine Genome Arrays and washed and stained with Affymetrix Wash&Stain Kit on an Affymetrix Fluidics Station 450
|
Scan protocol |
Affymetrix GeneChip Scanner 3000
|
Description |
endometrial tissue; ovariectomized cow
|
Data processing |
RMA Normalization
|
|
|
Submission date |
Jun 29, 2009 |
Last update date |
Mar 25, 2010 |
Contact name |
Stefan Krebs |
E-mail(s) |
krebs-st@lmb.uni-muenchen.de
|
Phone |
0049-89-2180 76715
|
Organization name |
Ludwig-Maximilian University
|
Department |
Gene Center
|
Lab |
Lafuga Genomics
|
Street address |
Feodor-Lynen-Str. 25
|
City |
Munich |
ZIP/Postal code |
81377 |
Country |
Germany |
|
|
Platform ID |
GPL2112 |
Series (1) |
GSE16880 |
Actions and interactions of progesterone and estrogen on transcriptome profiles of the bovine endometrium |
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