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Sample GSM423921 Query DataSets for GSM423921
Status Public on Mar 31, 2010
Title Wildtype (WT) or Cont sample 6
Sample type RNA
 
Source name left ventricle
Organism Mus musculus
Characteristics strain: mixed genetic background
age (weeks): 33.0
ef%: 78.3
sex: male
tissue: left ventricle
Extracted molecule total RNA
Extraction protocol RNA was extracted using an RNAeasy fibrous tissue mini kit (Qiagen), followed by clean-up and DNase I treatment in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent 2100 Bioanalyser.
Label biotin
Label protocol Biotinylated cRNAs were prepared with the Illumina TotalPrep RNA amplification kit.
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol with BeadStation 500 GX with scan settings at the default settings for Direct Hybridization.
Description 055-B
Data processing Raw expression data of the 24 mice from the Illumina BeadStudio software were processed using the lumi program (version 1.6.3), which is an implemented package designed for processing Illumina microarrays in R (version 2.8.0) [http://www.R-project.org.], the open source environment of statistical analysis. The raw intensities from each mouse sample were transformed using variance stabilization transformation (VST), which takes advantage of the multiple replicates available on the Illumina arrays.
 
Submission date Jun 30, 2009
Last update date Mar 31, 2010
Contact name Margot C LaPointe
E-mail(s) mlapoin1@hfhs.org
Phone 313-916-3076
Fax 313-916-1479
Organization name Henry Ford Hospital
Department Internal Medicine
Lab Hyp. and Vasc. Res. Div.
Street address 2799 W. Grand Blvd.
City Detroit
State/province MI
ZIP/Postal code 48202-2689
Country USA
 
Platform ID GPL6887
Series (1)
GSE16909 Gene expression profiling of a mouse model of dilated cardiomyopathy

Data table header descriptions
ID_REF
VALUE Variance stabilized data were normalized by quantile normalization method.

Data table
ID_REF VALUE
ILMN_2735294 9.92507019768129
ILMN_2417611 7.96747799743545
ILMN_2545897 8.7276794409857
ILMN_2762289 8.08907352847155
ILMN_1248788 8.32981584769617
ILMN_2707227 8.05863596096977
ILMN_2896528 13.5439661831823
ILMN_2721178 11.4636346979511
ILMN_1227723 10.4765369812172
ILMN_2458837 7.94826167412077
ILMN_3033922 11.0626383436376
ILMN_3092673 12.8698957597854
ILMN_1230777 11.9999397090868
ILMN_2730714 13.2507952038286
ILMN_1246069 15.0929494798482
ILMN_1232042 7.98241906406529
ILMN_1243193 10.4380752535219
ILMN_2524361 7.91109518261916
ILMN_1233188 8.41962174821134
ILMN_2543688 9.79530173754553

Total number of rows: 45281

Table truncated, full table size 1319 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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