|
| Status |
Public on Jan 01, 2020 |
| Title |
SCs from control rat5 [C5] |
| Sample type |
SRA |
| |
|
| Source name |
SCs from control
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain background: Sprague-Dawley rats
model: control rat
tissue: sciatic nerve
cell type: Schwann cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Schwann cell samples were randomly selected from control groups (n = 3) and diabetic groups (n = 3), rinsed with PBS twice and total RNA was isolated by using miRNeasy Mini Kit (Qiagen, Germany) according to the manufacturer’s instructions.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Illumina Xten software used for basecalling.
Transcriptome sequencing uses high-throughput sequencing technology to sequence the cDNA after total RNA reverse transcription, and obtains most transcripts of samples in this state.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to whole genome and FPKM were calculated using StringTie（Pertea et al., 2016)
Genome_build: rn6
Supplementary_files_format_and_content: FPKM values and counts.
|
| |
|
| Submission date |
Dec 31, 2019 |
| Last update date |
Jan 01, 2020 |
| Contact name |
cheng wang |
| E-mail(s) |
wangchenghust@hust.edu.cn
|
| Phone |
+86 18674017375
|
| Organization name |
Huazhong University of Science and Technology
|
| Department |
Union Hospital of Tongji Medical College
|
| Lab |
Department of Hand Surgery
|
| Street address |
No. 1277 Jiefang Avenue
|
| City |
Wuhan |
| ZIP/Postal code |
430022 |
| Country |
China |
| |
|
| Platform ID |
GPL24688 |
| Series (1) |
| GSE142785 |
RNA Sequencing Facilitates Quantitative Analysis of Schwann cells Transcriptomes |
|
| Relations |
| BioSample |
SAMN13703122 |
| SRA |
SRX7483496 |
