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Sample GSM424464 Query DataSets for GSM424464
Status Public on Sep 23, 2009
Title Dgcr8 KO replicate 2
Sample type RNA
 
Source name Embryonic Stem Cells
Organism Mus musculus
Characteristics cell type: embryonic stem cells
genetic modification: Dgcr8 knockout
Growth protocol Wild-type, dgcr8 knockout, and dicer1 knockout ES cells were plated on 60-mm plates in triplicate.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Gladstone Genomic Core facility standard protocols were used (http://www.gladstone.ucsf.edu/gladstone/site/genomicscore/section/1914).
 
Hybridization protocol Gladstone Genomic Core facility standard protocols were used (http://www.gladstone.ucsf.edu/gladstone/site/genomicscore/section/1914).
Scan protocol Gladstone Genomic Core facility standard protocols were used (http://www.gladstone.ucsf.edu/gladstone/site/genomicscore/section/1914).
Description Gene expression data from Dgcr8 knockout ES cells
Data processing Data were normalized using the GCRMA algorithm.
 
Submission date Jul 01, 2009
Last update date Sep 23, 2009
Contact name Robert Blelloch
E-mail(s) blellochr@stemcell.ucsf.edu
Phone 415-476-2838
Organization name University of California, San Francisco
Street address 513 Parnassus Avenue, Campus Box #0525
City San Francisco
State/province CA
ZIP/Postal code 94143
Country USA
 
Platform ID GPL6246
Series (1)
GSE16923 Expression data from wild-type, Dgcr8 knockout and Dicer knockout ES cells

Data table header descriptions
ID_REF
VALUE RMA normalized signal

Data table
ID_REF VALUE
10338001 12.00474
10338002 7.472782
10338003 10.6957
10338004 9.810118
10338005 2.425232
10338006 2.79234
10338007 3.479561
10338008 4.843608
10338009 9.661083
10338010 2.504559
10338011 6.791224
10338012 2.61263
10338013 2.228914
10338014 2.306805
10338015 2.285787
10338016 8.821213
10338017 13.23832
10338018 7.981737
10338019 6.27496
10338020 9.618604

Total number of rows: 35557

Table truncated, full table size 621 Kbytes.




Supplementary file Size Download File type/resource
GSM424464.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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