|
| Status |
Public on Jul 01, 2010 |
| Title |
ERK1/2 wild type EC, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Aortic EC isolated from mouse infected with lentivirus-egfp
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/BL6 (5 generations)
age: 4 Weeks
cell type: aortic endothelial cell
genotype: Erk1-/-; Erk2fl/fl + lentiviral-GFP
|
| Treatment protocol |
EC were grown in complete EC media (DMEM-F12+20% FBS+heparin+ECGS+PS) in a 37º incubator with 5% CO2.
|
| Growth protocol |
EC were isolated from mouse aorta
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA was hybridized for 16 hours at 44ºC on Mouse Exon 1.0 ST GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from EC with wild type ERK1/2
|
| Data processing |
RMA method was applied to the primary data to correct the technical bias and summarize gene expression values over probe-sets
|
| |
|
| Submission date |
Jul 06, 2009 |
| Last update date |
Jul 06, 2009 |
| Contact name |
Ruchika Srinivasan |
| E-mail(s) |
ruchika.srinivasan@osumc.edu
|
| Phone |
614-688-3825
|
| Fax |
614-688-4181
|
| Organization name |
The Ohio State University
|
| Street address |
Rm 840 W12th avenue
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43201 |
| Country |
USA |
| |
|
| Platform ID |
GPL6096 |
| Series (1) |
| GSE16967 |
Expression data from ERK1/2 null endothelial cells |
|
