|
| Status |
Public on Mar 21, 2012 |
| Title |
C2C12_3hr_differentiation_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
C2C12 myoblasts, differentiating, 3h
|
| Organism |
Mus musculus |
| Characteristics |
cell line: C2C12
time point: 3hr_differentiation
replicate: 2
|
| Treatment protocol |
Cells were differentiated by changing the medium to DMEM + 2% horse serum.
|
| Growth protocol |
C2C12 myoblasts were cultured in DMEM + 20% FBS before inducing differentiation by changing to DMEM + 2% horse serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
cRNA amplification and labeling with biotin were performed using Illlumina TotalPrep RNA amplification kit, manufactured by Ambion, Inc. (Austin, TX) according to the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol using the Illumina BeadArray Reader confocal scanner
|
| Description |
Gene expression data from differentiating C2C12 myoblasts grown in 2% horse serum
1562232001_B
|
| Data processing |
The data was processed using the FlexArray package and normalized using LUMI
|
| |
|
| Submission date |
Jul 10, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Saravanan Sundararajan |
| Organization name |
McGill University
|
| Department |
Human Genetics
|
| Lab |
Rob Sladek
|
| Street address |
740, Dr Penfield Ave
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3A 1A4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6105 |
| Series (2) |
| GSE17038 |
Expression Profiling of Early Myogenesis - Illumina Dataset |
| GSE17039 |
Expression Profiling of Early Myogenesis |
|
