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Sample GSM426677 Query DataSets for GSM426677
Status Public on Apr 02, 2010
Title SPMS, N14S
Sample type RNA
 
Source name blood, SPMS
Organism Homo sapiens
Characteristics ms subtype: SPMS
tissue: whole blood
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from the whole blood in PAXgene tubes. Total RNA quality was assessed and its concentration was measured using the Agilent RNA 6000 series II Nano kit (Agilent Technologies, CA). 250 ng total RNA from each sample was biotinylated and amplified using Illumina® TotalPrep RNA Amplification Kit (Ambion, TX). Total RNA was reverse transcribed to synthesize the first strand of cDNA, followed by a second strand synthesis.
Label biotin
Label protocol Double-stranded cDNA was then transcribed and amplified in vitro to synthesize biotin-labelled complementary mRNA (cRNA).
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description N14S
Data processing The data were normalised using cubic spline normalisation with Illumina beadstudio.

Non-normalized data is available in the supplementary file 'GSE17048_non-normalized_data.txt', which is linked to the Series GSE17048 record.
 
Submission date Jul 10, 2009
Last update date Apr 02, 2010
Contact name David Richmond Booth
E-mail(s) david_booth@wmi.usyd.edu.au
Phone 0061 2 98458498
Fax 0061 2 98913889
Organization name Westmead Millennium Institute
Department Immunology
Street address Darcy Rd
City Westmead
State/province NSW
ZIP/Postal code 2145
Country Australia
 
Platform ID GPL6947
Series (1)
GSE17048 Multiple Sclerosis Blood Cell mRNA Transcriptome

Data table header descriptions
ID_REF
VALUE cubic spline normalized value

Data table
ID_REF VALUE
ILMN_1762337 -4.167367
ILMN_1736007 17.84731
ILMN_2383229 -1.142694
ILMN_1671474 19.08529
ILMN_1772582 -3.576543
ILMN_1735698 -3.790443
ILMN_1653355 4.136366
ILMN_1787689 0.9461816
ILMN_1745607 -5.304598
ILMN_2136495 -6.836081
ILMN_1668111 -10.18193
ILMN_1735045 -7.47171
ILMN_1680754 5.085561
ILMN_2375184 -2.475133
ILMN_1755321 34.5293
ILMN_1698554 51.9143
ILMN_1814092 2.038013
ILMN_1760414 -1.837318
ILMN_2061446 51.26791
ILMN_1752884 -7.472741

Total number of rows: 37804

Table truncated, full table size 822 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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