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Sample GSM426697 Query DataSets for GSM426697
Status Public on Apr 02, 2010
Title PPMS, P044
Sample type RNA
 
Source name blood, PPMS
Organism Homo sapiens
Characteristics ms subtype: PPMS
tissue: whole blood
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from the whole blood in PAXgene tubes. Total RNA quality was assessed and its concentration was measured using the Agilent RNA 6000 series II Nano kit (Agilent Technologies, CA). 250 ng total RNA from each sample was biotinylated and amplified using Illumina® TotalPrep RNA Amplification Kit (Ambion, TX). Total RNA was reverse transcribed to synthesize the first strand of cDNA, followed by a second strand synthesis.
Label biotin
Label protocol Double-stranded cDNA was then transcribed and amplified in vitro to synthesize biotin-labelled complementary mRNA (cRNA).
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description P044
Data processing The data were normalised using cubic spline normalisation with Illumina beadstudio.

Non-normalized data is available in the supplementary file 'GSE17048_non-normalized_data.txt', which is linked to the Series GSE17048 record.
 
Submission date Jul 10, 2009
Last update date Apr 02, 2010
Contact name David Richmond Booth
E-mail(s) david_booth@wmi.usyd.edu.au
Phone 0061 2 98458498
Fax 0061 2 98913889
Organization name Westmead Millennium Institute
Department Immunology
Street address Darcy Rd
City Westmead
State/province NSW
ZIP/Postal code 2145
Country Australia
 
Platform ID GPL6947
Series (1)
GSE17048 Multiple Sclerosis Blood Cell mRNA Transcriptome

Data table header descriptions
ID_REF
VALUE cubic spline normalized value

Data table
ID_REF VALUE
ILMN_1762337 -10.3906
ILMN_1736007 1.490776
ILMN_2383229 4.309023
ILMN_1671474 9.634352
ILMN_1772582 -12.86565
ILMN_1735698 13.97799
ILMN_1653355 3.105065
ILMN_1787689 -10.38394
ILMN_1745607 2.490978
ILMN_2136495 -3.449869
ILMN_1668111 -6.219437
ILMN_1735045 -5.975399
ILMN_1680754 11.86494
ILMN_2375184 1.358204
ILMN_1755321 32.44602
ILMN_1698554 125.6071
ILMN_1814092 2.506302
ILMN_1760414 1.51544
ILMN_2061446 47.37346
ILMN_1752884 9.746701

Total number of rows: 37804

Table truncated, full table size 822 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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