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Sample GSM426714 Query DataSets for GSM426714
Status Public on Apr 02, 2010
Title PPMS, N15P
Sample type RNA
 
Source name blood, PPMS
Organism Homo sapiens
Characteristics ms subtype: PPMS
tissue: whole blood
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from the whole blood in PAXgene tubes. Total RNA quality was assessed and its concentration was measured using the Agilent RNA 6000 series II Nano kit (Agilent Technologies, CA). 250 ng total RNA from each sample was biotinylated and amplified using Illumina® TotalPrep RNA Amplification Kit (Ambion, TX). Total RNA was reverse transcribed to synthesize the first strand of cDNA, followed by a second strand synthesis.
Label biotin
Label protocol Double-stranded cDNA was then transcribed and amplified in vitro to synthesize biotin-labelled complementary mRNA (cRNA).
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description N15P
Data processing The data were normalised using cubic spline normalisation with Illumina beadstudio.

Non-normalized data is available in the supplementary file 'GSE17048_non-normalized_data.txt', which is linked to the Series GSE17048 record.
 
Submission date Jul 10, 2009
Last update date Apr 02, 2010
Contact name David Richmond Booth
E-mail(s) david_booth@wmi.usyd.edu.au
Phone 0061 2 98458498
Fax 0061 2 98913889
Organization name Westmead Millennium Institute
Department Immunology
Street address Darcy Rd
City Westmead
State/province NSW
ZIP/Postal code 2145
Country Australia
 
Platform ID GPL6947
Series (1)
GSE17048 Multiple Sclerosis Blood Cell mRNA Transcriptome

Data table header descriptions
ID_REF
VALUE cubic spline normalized value

Data table
ID_REF VALUE
ILMN_1762337 -12.46738
ILMN_1736007 8.851187
ILMN_2383229 3.051701
ILMN_1671474 20.77529
ILMN_1772582 1.302722
ILMN_1735698 -9.402886
ILMN_1653355 15.5213
ILMN_1787689 -0.3764844
ILMN_1745607 -3.123583
ILMN_2136495 11.79241
ILMN_1668111 -3.462394
ILMN_1735045 -0.1127114
ILMN_1680754 -9.929429
ILMN_2375184 -1.456099
ILMN_1755321 24.69627
ILMN_1698554 43.52154
ILMN_1814092 16.63343
ILMN_1760414 -0.2518131
ILMN_2061446 65.33071
ILMN_1752884 -5.68299

Total number of rows: 37804

Table truncated, full table size 822 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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