|
| Status |
Public on Mar 31, 2010 |
| Title |
AI Rat no DL-310 |
| Sample type |
RNA |
| |
|
| Source name |
Cardiac left ventricle
|
| Organism |
Rattus norvegicus |
| Characteristics |
gender: male
strain: Wistar
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from stored tissues using Trizol (Invitrogen), and further purified on column with RNeasy MinElute (Qiagen). RNA concentration and integrity were assessed by UV spectrophotometry (Nanodrop ND-1000, Thermo Scientific) and microfluidic electrophoresis (Bioanalyzer 2100, Agilent), respectively.
|
| Label |
biotin
|
| Label protocol |
Total RNA extracts were treated for double-stranded cDNA synthesis and labeling of cRNA with biotin according to the Eberwine method (Van Gelder et al 1990), using the Illumina TotalPrep RNA Amplification kit reagents and protocol (Ambion cat no IL1791).
|
| |
|
| Hybridization protocol |
The lyophilized biotin-labeled cRNA preparations were hybridized to BeadChip RatRef-12 microarrays (Illumina) according to Illumina’s protocol (11286340 rev. A), using 750 ng cRNA per array. After hybridization and washes, arrays were incubated in streptavidin-Cy3 solution
|
| Scan protocol |
Cy3 fluorescence signals were read on a BeadArray reader (Illumina).
|
| Description |
none
|
| Data processing |
Raw fluorescence data were processed and normalized with the lumi package (FlexArray microarray analysis software, version 1.3.1), using RMA background adjustment, VST variance stabilization, and Robust Spline Normalization algorithms.
|
| |
|
| Submission date |
Jul 10, 2009 |
| Last update date |
Dec 22, 2009 |
| Contact name |
Jacques Couet |
| E-mail(s) |
jacques.couet@med.ulaval.ca
|
| Organization name |
Centre de Recherche de l'Hopital Laval
|
| Street address |
2725 chemin Sainte-Foy
|
| City |
Quebec |
| ZIP/Postal code |
G1V4G5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6101 |
| Series (1) |
| GSE17050 |
Gene expression profiling in Wistar male rat left ventricle with chronic and severe aortic valve regurgitation |
|
