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Sample GSM427275 Query DataSets for GSM427275
Status Public on Mar 31, 2010
Title Ferret lung with A/California/07/2009 (H1N1) on Day 3, biological rep3
Sample type RNA
 
Source name Ferret
Organism Mustela putorius furo
Characteristics tissue: lung
virus strain: A/California/07/2009 (H1N1)
Treatment protocol Intranasal infection of ferrets with 10^6 EID50 A/California/07/2009 (H1N1) for the first experiment and 10^6 EID50 A/Brisbane/59/2007 (H1N1) for the second experiment. On Day 0 (before infection), Day 3 (post infection for the first experiment) or Day 2 (post infection for the second experiment), and Day 5, Day 7 and Day 14 (post infection for both experiments), ferrets were euthanized and lung tissue was excised and put in RNAlater (Ambion, Austin, TX) immediately.
Extracted molecule total RNA
Extraction protocol TRIzol extraction of total RNA from lung was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix Two-Cycle protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Canine Genome 2.0 Array in Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Description Gene expression data from ferret lung on Day 3 after infection
Data processing The data were analyzed with PLIER using Expression Console Version 1.1 (Affymetrix) default analysis settings.
 
Submission date Jul 14, 2009
Last update date Mar 31, 2010
Contact name Longsi Ran
E-mail(s) lran@uhnresearch.ca
Phone 416-581-7607
Organization name Toronto General Research Institute
Department Division of Experimental Therapeutics
Lab Dr. David J Kelvin
Street address 101 College Street
City Toronto
State/province ON
ZIP/Postal code M5G 1L7
Country Canada
 
Platform ID GPL3738
Series (1)
GSE17079 Early CCL2 and CXCL10 chemokine expression correlates with lung pathology in Pandemic H1N1 Influenza A infection

Data table header descriptions
ID_REF
VALUE PLIER-calculated Signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 56.16683
AFFX-BioB-5_at 57.33388
AFFX-BioB-M_at 78.34039
AFFX-BioC-3_at 194.4141
AFFX-BioC-5_at 159.3969
AFFX-BioDn-3_at 608.1444
AFFX-BioDn-5_at 426.3602
AFFX-Cfa-actin-3_s_at 3178.638
AFFX-Cfa-actin-5_at 725.0672
AFFX-Cfa-actin-5_s_at 1439.377
AFFX-Cfa-actin-M_x_at 3776.374
AFFX-Cfa-ef1a-3_x_at 4053.255
AFFX-Cfa-ef1a-5_at 2.998104
AFFX-Cfa-ef1a-M_x_at 2398.072
AFFX-Cfa-gapdh-3_x_at 103.6863
AFFX-Cfa-gapdh-5_x_at 55.85527
AFFX-Cfa-gapdh-M_x_at 62.41256
AFFX-Cfa-r2-Bs-dap-3_at 1.817755
AFFX-Cfa-r2-Bs-dap-5_at 8.548989
AFFX-Cfa-r2-Bs-dap-M_at 1.367218

Total number of rows: 43035

Table truncated, full table size 1232 Kbytes.




Supplementary file Size Download File type/resource
GSM427275.CEL.gz 3.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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