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Sample GSM4283825 Query DataSets for GSM4283825
Status Public on Apr 10, 2021
Title GRKO_Gasttoc_1
Sample type RNA
 
Source name GRKO_gastrocnemius muscle
Organism Mus musculus
Characteristics strain background: C57/Bl6J
genotype/variation: GR KO
tissue: gastrocnemius muscle
Growth protocol Mice were maintained in a temperature- and humidity-controlled animal facility, with a 12 hours light/dark cycle.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description 125G
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Jan 24, 2020
Last update date Apr 10, 2021
Contact name Scott Jelinsky
E-mail(s) Scott.Jelinsky@pfizer.com
Phone 617-674-7272
Organization name Pfizer
Department Inflammation and Immunology
Lab Computational Precision Medicine
Street address 610 Main Street
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platform ID GPL1261
Series (1)
GSE144228 The glucocorticoid receptor coordinates myofiber-selective transcriptional networks

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1415670_at 289
1415671_at 448
1415672_at 565
1415673_at 115
1415674_a_at 151
1415675_at 97
1415676_a_at 1761
1415677_at 79
1415678_at 1893
1415679_at 1296
1415680_at 153
1415681_at 463
1415682_at 179
1415683_at 873
1415684_at 77
1415685_at 242
1415686_at 297
1415687_a_at 2651
1415688_at 1388
1415689_s_at 122

Total number of rows: 45101

Table truncated, full table size 633 Kbytes.




Supplementary file Size Download File type/resource
GSM4283825_125G_121208_F3.cel.gz 5.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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