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Sample GSM4283826 Query DataSets for GSM4283826
Status Public on Apr 10, 2021
Title GRKO_Gasttoc_2
Sample type RNA
 
Source name GRKO_gastrocnemius muscle
Organism Mus musculus
Characteristics strain background: C57/Bl6J
genotype/variation: GR KO
tissue: gastrocnemius muscle
Growth protocol Mice were maintained in a temperature- and humidity-controlled animal facility, with a 12 hours light/dark cycle.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description 126G
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Jan 24, 2020
Last update date Apr 10, 2021
Contact name Scott Jelinsky
E-mail(s) Scott.Jelinsky@pfizer.com
Phone 617-674-7272
Organization name Pfizer
Department Inflammation and Immunology
Lab Computational Precision Medicine
Street address 610 Main Street
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platform ID GPL1261
Series (1)
GSE144228 The glucocorticoid receptor coordinates myofiber-selective transcriptional networks

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1415670_at 263
1415671_at 559
1415672_at 369
1415673_at 83
1415674_a_at 173
1415675_at 123
1415676_a_at 2190
1415677_at 46
1415678_at 1728
1415679_at 1868
1415680_at 142
1415681_at 579
1415682_at 155
1415683_at 654
1415684_at 33
1415685_at 268
1415686_at 156
1415687_a_at 3205
1415688_at 1043
1415689_s_at 122

Total number of rows: 45101

Table truncated, full table size 632 Kbytes.




Supplementary file Size Download File type/resource
GSM4283826_126G_121208_E6.cel.gz 5.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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