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Status |
Public on Apr 10, 2021 |
Title |
GRKO_Gasttoc_2 |
Sample type |
RNA |
|
|
Source name |
GRKO_gastrocnemius muscle
|
Organism |
Mus musculus |
Characteristics |
strain background: C57/Bl6J genotype/variation: GR KO tissue: gastrocnemius muscle
|
Growth protocol |
Mice were maintained in a temperature- and humidity-controlled animal facility, with a 12 hours light/dark cycle.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Description |
126G
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
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|
|
Submission date |
Jan 24, 2020 |
Last update date |
Apr 10, 2021 |
Contact name |
Scott Jelinsky |
E-mail(s) |
Scott.Jelinsky@pfizer.com
|
Phone |
617-674-7272
|
Organization name |
Pfizer
|
Department |
Inflammation and Immunology
|
Lab |
Computational Precision Medicine
|
Street address |
610 Main Street
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02139 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE144228 |
The glucocorticoid receptor coordinates myofiber-selective transcriptional networks |
|