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Sample GSM4283828 Query DataSets for GSM4283828
Status Public on Apr 10, 2021
Title GRKO_Gasttoc_4
Sample type RNA
 
Source name GRKO_gastrocnemius muscle
Organism Mus musculus
Characteristics strain background: C57/Bl6J
genotype/variation: GR KO
tissue: gastrocnemius muscle
Growth protocol Mice were maintained in a temperature- and humidity-controlled animal facility, with a 12 hours light/dark cycle.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description 130G
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Jan 24, 2020
Last update date Apr 10, 2021
Contact name Scott Jelinsky
E-mail(s) Scott.Jelinsky@pfizer.com
Phone 617-674-7272
Organization name Pfizer
Department Inflammation and Immunology
Lab Computational Precision Medicine
Street address 610 Main Street
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platform ID GPL1261
Series (1)
GSE144228 The glucocorticoid receptor coordinates myofiber-selective transcriptional networks

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1415670_at 316
1415671_at 607
1415672_at 393
1415673_at 108
1415674_a_at 265
1415675_at 125
1415676_a_at 2557
1415677_at 72
1415678_at 1527
1415679_at 2578
1415680_at 143
1415681_at 667
1415682_at 133
1415683_at 708
1415684_at 59
1415685_at 237
1415686_at 197
1415687_a_at 4211
1415688_at 934
1415689_s_at 102

Total number of rows: 45101

Table truncated, full table size 630 Kbytes.




Supplementary file Size Download File type/resource
GSM4283828_130G_121208_B8.cel.gz 5.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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