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Sample GSM428970 Query DataSets for GSM428970
Status Public on Feb 21, 2010
Title 3h CH-treated EpiSCs
Sample type RNA
Source name cell culture from E3 epiblast stem cells
Organism Mus musculus
Characteristics cell line: E3
Treatment protocol see below
Growth protocol Grown on inactivated mouse embryonic fibroblasts (CF1) in KSR medium supplemented with 5 ng/ml FGF2. At semiconfluence change to KSR medium with 3uM CHIR99021 for 3h.
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit with on-column DNA digestion
Label Cy-3
Label protocol Ambion TotalPrep RNA Amplification Kit. RNA in put: 400ng, IVT: 12h
Hybridization protocol According to manufacturer's instructions. Hybridization time: 18h
Scan protocol Illumina iScan system using default settings
Description N/A
Data processing Cubic Spline normalization with background subtraction in BeadStudio. Values below 50% of the detection threshold intensity (detection p value = 0.010) were set to "Null" (= not expressed / detectable).
Submission date Jul 16, 2009
Last update date Feb 20, 2010
Contact name Boris Greber
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Street address Röntgenstraße 20
City Münster
ZIP/Postal code 48149
Country Germany
Platform ID GPL6885
Series (2)
GSE17133 3h pathway stimulation / inhibition experiment in epiblast stem cells
GSE17202 Expression profiles of epiblast stem cells and embryonic stem cells

Data table header descriptions
VALUE normalized and background subtracted intensity value

Data table
ILMN_1212607 null
ILMN_1212612 null
ILMN_1212619 18
ILMN_1212628 null
ILMN_1212632 33
ILMN_1212636 2590
ILMN_1212637 278
ILMN_1212645 13
ILMN_1212648 93
ILMN_1212653 7
ILMN_1212672 230
ILMN_1212682 null
ILMN_1212683 null
ILMN_1212685 null
ILMN_1212692 72
ILMN_1212693 20
ILMN_1212695 null
ILMN_1212698 null
ILMN_1212702 9
ILMN_1212703 null

Total number of rows: 25697

Table truncated, full table size 430 Kbytes.

Supplementary file Size Download File type/resource
GSM428970_T3_3h_CH_raw.txt.gz 376.3 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table

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