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Sample GSM428974 Query DataSets for GSM428974
Status Public on Feb 21, 2010
Title 12h untreated EpiSCs
Sample type RNA
 
Source name cell culture from E3 epiblast stem cells
Organism Mus musculus
Characteristics cell line: E3
Treatment protocol see below
Growth protocol Grown on inactivated mouse embryonic fibroblasts (CF1) in KSR medium supplemented with 5 ng/ml FGF2. At semiconfluence change to KSR medium without supplements for 12h.
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit with on-column DNA digestion
Label Cy-3
Label protocol Ambion TotalPrep RNA Amplification Kit. RNA in put: 400ng, IVT: 12h
 
Hybridization protocol According to manufacturer's instructions. Hybridization time: 18h
Scan protocol Illumina iScan system using default settings
Description N/A
Data processing Cubic Spline normalization with background subtraction in BeadStudio. Values below 50% of the detection threshold intensity (detection p value = 0.010) were set to "Null" (= not expressed / detectable).
 
Submission date Jul 16, 2009
Last update date Feb 20, 2010
Contact name Boris Greber
E-mail(s) boris.greber@mpi-muenster.mpg.de
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Street address Röntgenstraße 20
City Münster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (2)
GSE17134 12h pathway stimulation / inhibition experiment in epiblast stem cells
GSE17202 Expression profiles of epiblast stem cells and embryonic stem cells

Data table header descriptions
ID_REF
VALUE normalized and background subtracted intensity value

Data table
ID_REF VALUE
ILMN_1212607 null
ILMN_1212612 null
ILMN_1212619 11
ILMN_1212628 null
ILMN_1212632 32
ILMN_1212636 2262
ILMN_1212637 264
ILMN_1212645 40
ILMN_1212648 75
ILMN_1212653 10
ILMN_1212672 206
ILMN_1212682 13
ILMN_1212683 null
ILMN_1212685 null
ILMN_1212692 196
ILMN_1212693 null
ILMN_1212695 null
ILMN_1212698 null
ILMN_1212702 9
ILMN_1212703 17

Total number of rows: 25697

Table truncated, full table size 434 Kbytes.




Supplementary file Size Download File type/resource
GSM428974_T4_12h_UT_raw.txt.gz 379.2 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table

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