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Sample GSM428979 Query DataSets for GSM428979
Status Public on Feb 21, 2010
Title 12h FL-treated ES cells
Sample type RNA
Source name cell culture from OG2 embryonic stem cells
Organism Mus musculus
Characteristics cell line: OG2
Treatment protocol see below
Growth protocol Grown on gelatin in KSR medium supplemented with 1uM PD0325901, 3uM CHIR99021, and 1000 U/ml LIF. Then switch to 10 ng/ml FGF2 + LIF for 12h. Cells were harvested directly on the culture dish.
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit with on-column DNA digestion
Label Cy-3
Label protocol Ambion TotalPrep RNA Amplification Kit. RNA in put: 400ng, IVT: 12h
Hybridization protocol According to manufacturer's instructions. Hybridization time: 18h
Scan protocol Illumina iScan system using default settings
Description N/A
Data processing Cubic Spline normalization with background subtraction in BeadStudio. Values below 50% of the detection threshold intensity (detection p value = 0.010) were set to "Null" (= not expressed / detectable).
Submission date Jul 16, 2009
Last update date Feb 20, 2010
Contact name Boris Greber
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Street address Röntgenstraße 20
City Münster
ZIP/Postal code 48149
Country Germany
Platform ID GPL6885
Series (2)
GSE17136 12h pathway stimulation / inhibition experiment in mouse ES cells
GSE17202 Expression profiles of epiblast stem cells and embryonic stem cells

Data table header descriptions
VALUE normalized and background subtracted intensity value

Data table
ILMN_1212607 8
ILMN_1212612 null
ILMN_1212619 19
ILMN_1212628 null
ILMN_1212632 55
ILMN_1212636 2200
ILMN_1212637 230
ILMN_1212645 10
ILMN_1212648 173
ILMN_1212653 6
ILMN_1212672 140
ILMN_1212682 null
ILMN_1212683 null
ILMN_1212685 null
ILMN_1212692 25
ILMN_1212693 6
ILMN_1212695 null
ILMN_1212698 null
ILMN_1212702 null
ILMN_1212703 13

Total number of rows: 25697

Table truncated, full table size 431 Kbytes.

Supplementary file Size Download File type/resource
GSM428979_T5_12h_FL_raw.txt.gz 376.6 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table

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