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Sample GSM428980 Query DataSets for GSM428980
Status Public on Feb 21, 2010
Title 12h PJ-treated ES cells
Sample type RNA
 
Source name cell culture from OG2 embryonic stem cells
Organism Mus musculus
Characteristics cell line: OG2
Treatment protocol see below
Growth protocol Grown on gelatin in KSR medium supplemented with 1uM PD0325901, 3uM CHIR99021, and 1000 U/ml LIF. Then switch to PD + 1uM JAK inhibitor I for 12h. Cells were harvested directly on the culture dish.
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit with on-column DNA digestion
Label Cy-3
Label protocol Ambion TotalPrep RNA Amplification Kit. RNA in put: 400ng, IVT: 12h
 
Hybridization protocol According to manufacturer's instructions. Hybridization time: 18h
Scan protocol Illumina iScan system using default settings
Description N/A
Data processing Cubic Spline normalization with background subtraction in BeadStudio. Values below 50% of the detection threshold intensity (detection p value = 0.010) were set to "Null" (= not expressed / detectable).
 
Submission date Jul 16, 2009
Last update date Feb 20, 2010
Contact name Boris Greber
E-mail(s) boris.greber@mpi-muenster.mpg.de
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Street address Röntgenstraße 20
City Münster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (2)
GSE17136 12h pathway stimulation / inhibition experiment in mouse ES cells
GSE17202 Expression profiles of epiblast stem cells and embryonic stem cells

Data table header descriptions
ID_REF
VALUE normalized and background subtracted intensity value

Data table
ID_REF VALUE
ILMN_1212607 8
ILMN_1212612 null
ILMN_1212619 16
ILMN_1212628 null
ILMN_1212632 49
ILMN_1212636 1892
ILMN_1212637 296
ILMN_1212645 null
ILMN_1212648 207
ILMN_1212653 11
ILMN_1212672 128
ILMN_1212682 null
ILMN_1212683 null
ILMN_1212685 null
ILMN_1212692 27
ILMN_1212693 null
ILMN_1212695 null
ILMN_1212698 null
ILMN_1212702 null
ILMN_1212703 7

Total number of rows: 25697

Table truncated, full table size 431 Kbytes.




Supplementary file Size Download File type/resource
GSM428980_T5_12h_PJ_raw.txt.gz 377.9 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table

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