|
| Status |
Public on Jan 15, 2010 |
| Title |
Wild-type cell line 2, replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Tumor-derived cell line 2, wild-type STAT3
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Wild-type STAT3
|
| Treatment protocol |
none
|
| Growth protocol |
Cells were cultured in RPMI 1360 supplemented with FCS 20%, 2 mM Glutamax, 100 U/ml Pennicillin, 100 ug/ml Streptomycin and mainteined at 37°C in a 5% CO2 atmosphere
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol (Invitrogen) followed by purification on a Qiagen RNAeasy midi column
|
| Label |
biotin
|
| Label protocol |
RNA amplification and labelling were performed using the Ambion Illumina TotalPrep RNA Amplification Kit
|
| |
|
| Hybridization protocol |
For the hybridization step the samples are applied to the arrays and assembled into the BeadChip Hyb Chamber. The BeadChip is hybridized at 58°C overnight, and then washed the following day.
|
| Scan protocol |
Scanning was performed using Illumina Beadarray scanner
|
| Description |
none
|
| Data processing |
Raw expression data were obtained with BeadStudio, version 3.1.7. Normalization was performed with the lumi package of Bioconductor and default parameters
|
| |
|
| Submission date |
Jul 17, 2009 |
| Last update date |
Jan 12, 2010 |
| Contact name |
Paolo Provero |
| E-mail(s) |
paolo.provero@unito.it
|
| Organization name |
University of Turin
|
| Department |
Molecular Biotechnology and Health Sciences
|
| Street address |
Via Nizza 52
|
| City |
Torino |
| ZIP/Postal code |
I-10100 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE17182 |
Gene expression profiling of tumor cell lines with constitutively active STAT3 |
|
