|
| Status |
Public on Apr 01, 2010 |
| Title |
HCR_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Rat gastrocnemius muscle
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: high intrinsic aerobic capacity runners
generation: 18
gender: female
tissue: musculus gastrocnemius
age: 8 months
|
| Growth protocol |
Artificial breeding from a genetically heterogeous rat population (N:NIH stock) was used to produce rat strains differing in inherent aerobic capacity. The procedure was previously described in detail (Koch and Britton, Physiol Genomics. 2001; 5:45-52, PMID: 11161005).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from gastrocnemius muscle using Trizol Reagent (Invitrogen, Carlsbad, USA) according to the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared with the Illumina RNA TotalPrep Amplification kit (Ambion, # IL1791).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 4
RAT 8
|
| Data processing |
The data were normalized using quantile normalization from the affy package of Bioconductor (www.bioconductor.org) in R statistical programming language.
|
| |
|
| Submission date |
Jul 20, 2009 |
| Last update date |
Dec 23, 2009 |
| Contact name |
Heikki Kainulainen |
| E-mail(s) |
heikki.kainulainen@sport.jyu.fi
|
| Organization name |
University of Jyväskylä
|
| Department |
Department of Biology of Physical Activity
|
| Street address |
Seminaarinmäki
|
| City |
Jyväskylä |
| ZIP/Postal code |
FI-40014 |
| Country |
Finland |
| |
|
| Platform ID |
GPL6101 |
| Series (1) |
| GSE17190 |
Skeletal muscle characteristics of inherited low and high aerobic exercise capacity |
|
