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Sample GSM429933 Query DataSets for GSM429933
Status Public on Apr 01, 2010
Title LCR_rep7
Sample type RNA
 
Source name Rat gastrocnemius muscle
Organism Rattus norvegicus
Characteristics strain: low intrinsic aerobic capacity runners
generation: 18
gender: female
tissue: musculus gastrocnemius
age: 8 months
Growth protocol Artificial breeding from a genetically heterogeous rat population (N:NIH stock) was used to produce rat strains differing in inherent aerobic capacity. The procedure was previously described in detail (Koch and Britton, Physiol Genomics. 2001; 5:45-52, PMID: 11161005).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from gastrocnemius muscle using Trizol Reagent (Invitrogen, Carlsbad, USA) according to the manufacturer's protocol.
Label biotin
Label protocol Biotinylated cRNA was prepared with the Illumina RNA TotalPrep Amplification kit (Ambion, # IL1791).
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 7
RAT 13
Data processing The data were normalized using quantile normalization from the affy package of Bioconductor (www.bioconductor.org) in R statistical programming language.
 
Submission date Jul 20, 2009
Last update date Dec 23, 2009
Contact name Heikki Kainulainen
E-mail(s) heikki.kainulainen@sport.jyu.fi
Organization name University of Jyväskylä
Department Department of Biology of Physical Activity
Street address Seminaarinmäki
City Jyväskylä
ZIP/Postal code FI-40014
Country Finland
 
Platform ID GPL6101
Series (1)
GSE17190 Skeletal muscle characteristics of inherited low and high aerobic exercise capacity

Data table header descriptions
ID_REF
VALUE quantile normalized signal intensity

Data table
ID_REF VALUE
ILMN_1530370 67.37677083
ILMN_1530315 66.82347667
ILMN_1530343 76.47566292
ILMN_1530298 72.44786958
ILMN_1530312 79.10422875
ILMN_1530206 78.99734375
ILMN_1530174 81.44636375
ILMN_1530328 72.86834042
ILMN_1530537 68.63016667
ILMN_1530412 76.173565
ILMN_1530540 73.85628125
ILMN_1530418 74.86383792
ILMN_1530355 78.57651167
ILMN_1530438 83.94726667
ILMN_1530180 98.386215
ILMN_1530219 87.72678333
ILMN_1530535 86.92149833
ILMN_1530429 78.25144083
ILMN_1530449 75.2248725
ILMN_1530434 69.83935542

Total number of rows: 22517

Table truncated, full table size 543 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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