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Sample GSM429944 Query DataSets for GSM429944
Status Public on Apr 01, 2010
Title HCR_rep12
Sample type RNA
 
Source name Rat gastrocnemius muscle
Organism Rattus norvegicus
Characteristics strain: high intrinsic aerobic capacity runners
generation: 18
gender: female
tissue: musculus gastrocnemius
age: 8 months
Growth protocol Artificial breeding from a genetically heterogeous rat population (N:NIH stock) was used to produce rat strains differing in inherent aerobic capacity. The procedure was previously described in detail (Koch and Britton, Physiol Genomics. 2001; 5:45-52, PMID: 11161005).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from gastrocnemius muscle using Trizol Reagent (Invitrogen, Carlsbad, USA) according to the manufacturer's protocol.
Label biotin
Label protocol Biotinylated cRNA was prepared with the Illumina RNA TotalPrep Amplification kit (Ambion, # IL1791).
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 12
RAT 24
Data processing The data were normalized using quantile normalization from the affy package of Bioconductor (www.bioconductor.org) in R statistical programming language.
 
Submission date Jul 20, 2009
Last update date Dec 23, 2009
Contact name Heikki Kainulainen
E-mail(s) heikki.kainulainen@sport.jyu.fi
Organization name University of Jyväskylä
Department Department of Biology of Physical Activity
Street address Seminaarinmäki
City Jyväskylä
ZIP/Postal code FI-40014
Country Finland
 
Platform ID GPL6101
Series (1)
GSE17190 Skeletal muscle characteristics of inherited low and high aerobic exercise capacity

Data table header descriptions
ID_REF
VALUE quantile normalized signal intensity

Data table
ID_REF VALUE
ILMN_1530370 69.6838125
ILMN_1530315 74.1071375
ILMN_1530343 75.82578208
ILMN_1530298 77.65614583
ILMN_1530312 72.08959708
ILMN_1530206 78.09869667
ILMN_1530174 80.51320292
ILMN_1530328 74.11140375
ILMN_1530537 74.9369675
ILMN_1530412 76.37147583
ILMN_1530540 71.82446542
ILMN_1530418 75.75870167
ILMN_1530355 79.54461958
ILMN_1530438 96.54481708
ILMN_1530180 89.31890125
ILMN_1530219 93.27837042
ILMN_1530535 92.76717167
ILMN_1530429 74.79961833
ILMN_1530449 73.42399208
ILMN_1530434 76.076865

Total number of rows: 22517

Table truncated, full table size 543 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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