|
| Status |
Public on Feb 20, 2020 |
| Title |
control-6 |
| Sample type |
RNA |
| |
|
| Source name |
embryos
|
| Organism |
Danio rerio |
| Characteristics |
treatment: control
strain: Danio rerio ABTL strain
|
| Treatment protocol |
Control vs. ethanol-treated zebrafish embryos that were treated from 2 to 4.5 hours postfertilization prior to total RNA being isolated at 4.5 hours postfertilization.
|
| Growth protocol |
Both treatment groups were grown in embryo medium at 28.5 degrees C, but ethanol-treated zebrafish embryos that grown in 100 mM ethanol from 2 to 4.5 hours postfertilization.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from pools of 20 control and 20 ethanol-treated embryos using TRIzol reagent (Sigma, St Louis, MO, USA). Seven independent experiments were done. RNA samples were purified by passing through the Qiagen RNAeasy column (Cat. No. 74104).
|
| Label |
biotin
|
| Label protocol |
Standard protocol for Affymetrix 3' IVT Express kit using 100ng of total RNA
|
| |
|
| Hybridization protocol |
hybridized for 17h washed and stained on the Affymetrix Fluidics station
|
| Scan protocol |
Scanned using the Affymetrix 3000 scanner. Data was collected using GCOS software from Affymetrix.
|
| Data processing |
Affymetrix Gene Expression console was used to generate MAS5 signals, detection calls and detection p-values
|
| |
|
| Submission date |
Feb 19, 2020 |
| Last update date |
Feb 20, 2020 |
| Contact name |
James A Marrs |
| E-mail(s) |
jmarrs@iupui.edu
|
| Organization name |
Indiana University-Purdue University Indianapolis
|
| Department |
Biology
|
| Lab |
SL328
|
| Street address |
723 West Michigan Street
|
| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46202 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE145574 |
Embryonic ethanol exposure alters expression of sox2 and other early transcripts in zebrafish, producing gastrulation defects |
|
