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Sample GSM432899 Query DataSets for GSM432899
Status Public on Jul 24, 2009
Title DBA d18 (120msn)
Sample type RNA
 
Source name DBA mouse treated with pristane for 18 days
Organism Mus musculus
Characteristics agent: pristane
time: 18 days
strain: DBA
tissue: mesentery
Treatment protocol Using institutionally approved animal protocols, mice were given 0.5 ml TMPD intraperitoneally, and grow for 3 and 18 days. Animals untreated were prepared at the same time and used as the control
Extracted molecule total RNA
Extraction protocol total RNA was extracted from mesentery of experimental/control animals, using TRIzol (Invitrogen), and followed by a cleanup using Qiagen Rneasy kit.
Label biotin
Label protocol Labeled aRNA prepared from 1 µg of RNA using MessageAmpII aRNA Amplification kit ( Ambion) following manufactory instruction.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on mouse genome 430 2.0 array chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Genechip Scanner 3000.
Description no additional information
Data processing The data were analyzed with GeneChip® Operating Software (GCOS) using MAS5 Statistical algorithm.
The data in the matrix table are normalized by global scaling, and the mean target signal was set to 500.
 
Submission date Jul 24, 2009
Last update date Aug 28, 2018
Contact name Ke Zhang
E-mail(s) zhangk@mail.nih.gov
Phone 301-435-7250
Organization name NCI
Lab LCBG
Street address 37 Convent Dr.
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL1261
Series (1)
GSE17297 Mndal, a new interferon-inducible family member, suppresses cell growth and may modify plasmacytoma susceptibility
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 425.7
AFFX-BioB-M_at 725.2
AFFX-BioB-3_at 412.9
AFFX-BioC-5_at 1203.5
AFFX-BioC-3_at 1632.6
AFFX-BioDn-5_at 2494
AFFX-BioDn-3_at 6228.9
AFFX-CreX-5_at 13181.8
AFFX-CreX-3_at 16063
AFFX-DapX-5_at 10.1
AFFX-DapX-M_at 20.8
AFFX-DapX-3_at 26.4
AFFX-LysX-5_at 1.4
AFFX-LysX-M_at 5.3
AFFX-LysX-3_at 4.3
AFFX-PheX-5_at 2.8
AFFX-PheX-M_at 3.3
AFFX-PheX-3_at 16.8
AFFX-ThrX-5_at 7.7
AFFX-ThrX-M_at 16.5

Total number of rows: 45101

Table truncated, full table size 740 Kbytes.




Supplementary file Size Download File type/resource
GSM432899.CEL.gz 3.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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