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Sample GSM432916 Query DataSets for GSM432916
Status Public on Jul 24, 2009
Title DBA d3 (183msn)
Sample type RNA
Source name DBA mouse treated with pristane for 3 days
Organism Mus musculus
Characteristics agent: pristane
time: 3 days
strain: DBA
tissue: mesentery
Treatment protocol Using institutionally approved animal protocols, mice were given 0.5 ml TMPD intraperitoneally, and grow for 3 and 18 days. Animals untreated were prepared at the same time and used as the control
Extracted molecule total RNA
Extraction protocol total RNA was extracted from mesentery of experimental/control animals, using TRIzol (Invitrogen), and followed by a cleanup using Qiagen Rneasy kit.
Label biotin
Label protocol Labeled aRNA prepared from 1 µg of RNA using MessageAmpII aRNA Amplification kit ( Ambion) following manufactory instruction.
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on mouse genome 430 2.0 array chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Genechip Scanner 3000.
Description no additional information
Data processing The data were analyzed with GeneChip® Operating Software (GCOS) using MAS5 Statistical algorithm.
The data in the matrix table are normalized by global scaling, and the mean target signal was set to 500.
Submission date Jul 24, 2009
Last update date Aug 28, 2018
Contact name Ke Zhang
Phone 301-435-7250
Organization name NCI
Street address 37 Convent Dr.
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platform ID GPL1261
Series (1)
GSE17297 Mndal, a new interferon-inducible family member, suppresses cell growth and may modify plasmacytoma susceptibility
Reanalyzed by GSE119085

Data table header descriptions
VALUE MAS5.0 signal intensity

Data table
AFFX-BioB-5_at 482.4
AFFX-BioB-M_at 858.3
AFFX-BioB-3_at 574.3
AFFX-BioC-5_at 1451.4
AFFX-BioC-3_at 1679.7
AFFX-BioDn-5_at 3518.2
AFFX-BioDn-3_at 6647.1
AFFX-CreX-5_at 17700.3
AFFX-CreX-3_at 20120.8
AFFX-DapX-5_at 16.4
AFFX-DapX-M_at 3.5
AFFX-DapX-3_at 4.3
AFFX-LysX-5_at 6.7
AFFX-LysX-M_at 4
AFFX-LysX-3_at 15.7
AFFX-PheX-5_at 2.1
AFFX-PheX-M_at 1.1
AFFX-PheX-3_at 35.3
AFFX-ThrX-5_at 4.2
AFFX-ThrX-M_at 22.1

Total number of rows: 45101

Table truncated, full table size 736 Kbytes.

Supplementary file Size Download File type/resource
GSM432916.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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