|
| Status |
Public on Jul 24, 2009 |
| Title |
DBA d3 (184msn) |
| Sample type |
RNA |
| |
|
| Source name |
DBA mouse treated with pristane for 3 days
|
| Organism |
Mus musculus |
| Characteristics |
agent: pristane
time: 3 days
strain: DBA
tissue: mesentery
|
| Treatment protocol |
Using institutionally approved animal protocols, mice were given 0.5 ml TMPD intraperitoneally, and grow for 3 and 18 days. Animals untreated were prepared at the same time and used as the control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted from mesentery of experimental/control animals, using TRIzol (Invitrogen), and followed by a cleanup using Qiagen Rneasy kit.
|
| Label |
biotin
|
| Label protocol |
Labeled aRNA prepared from 1 µg of RNA using MessageAmpII aRNA Amplification kit ( Ambion) following manufactory instruction.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on mouse genome 430 2.0 array chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Genechip Scanner 3000.
|
| Description |
no additional information
|
| Data processing |
The data were analyzed with GeneChip® Operating Software (GCOS) using MAS5 Statistical algorithm.
The data in the matrix table are normalized by global scaling, and the mean target signal was set to 500.
|
| |
|
| Submission date |
Jul 24, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Ke Zhang |
| E-mail(s) |
zhangk@mail.nih.gov
|
| Phone |
301-435-7250
|
| Organization name |
NCI
|
| Lab |
LCBG
|
| Street address |
37 Convent Dr.
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE17297 |
Mndal, a new interferon-inducible family member, suppresses cell growth and may modify plasmacytoma susceptibility |
|
| Relations |
| Reanalyzed by |
GSE119085 |
