|
| Status |
Public on Jul 29, 2009 |
| Title |
Lymphoblastoid cell line control 1 |
| Sample type |
RNA |
| |
|
| Source name |
Lymphoblastoid cell line
|
| Organism |
Homo sapiens |
| Characteristics |
genotype: wild type KDM5C
cell line: Lymphoblastoid
disease state: normal control
|
| Treatment protocol |
No treatments were performed
|
| Growth protocol |
Patient and control lymphoblastoid cell lines were grown under normal conditions (in RPMI with 10%FCS, Glutamine and Pen/Strep) under 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent. RNA quality was checked with agarose gels.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit from Ambion
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
n/a
|
| Data processing |
BeadStudio 2.0 was used for processing the raw data and the Rank Invariant method was used for normalisation
|
| |
|
| Submission date |
Jul 28, 2009 |
| Last update date |
Jul 28, 2009 |
| Contact name |
Lars Riff Jensen |
| E-mail(s) |
jensen@molgen.mpg.de
|
| Phone |
(+49) 030 8413 1253
|
| Fax |
(+49) 030 8413 1394
|
| Organization name |
Max Planck Institute
|
| Department |
Human Molecular Genetics
|
| Lab |
Familial Cognitive Disorders
|
| Street address |
Ihnestrasse 73
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6097 |
| Series (1) |
| GSE17369 |
A gene expression fingerprint in mental retardation reflects disease causing defects in the histone demethylase KDM5 |
|
