|
| Status |
Public on Feb 05, 2010 |
| Title |
NT-LE-rep.2 |
| Sample type |
RNA |
| |
|
| Source name |
EpiSC from the epiblast of a somatic cell nuclear transfer embryo
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6D2
embryo stage: pre-streak/early streak
embryo morphology: Large Extra-embryonic ectoderm
|
| Treatment protocol |
none
|
| Growth protocol |
EpiSC were maintained on FBS coated dishes in Completely Defined Medium complemented with 20 ng/mL activine A (R&D) and 12 ng/mL bFgF (R&D) according to Brons et al., 2007.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the QIAGEN RNeasy minikit followed by DNAse I treatment in accordance with the manufacturer instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
n/a
|
| Data processing |
After exported from BeadStudio, data were normalised using lumiExpresso provided in lumi R package
|
| |
|
| Submission date |
Jul 29, 2009 |
| Last update date |
Dec 23, 2009 |
| Contact name |
Julien MARUOTTI |
| E-mail(s) |
maruotti@inapg.fr
|
| Organization name |
INRA
|
| Lab |
BDR-Bat 230
|
| Street address |
Domaine de Vilvert
|
| City |
JOUY EN JOSAS |
| ZIP/Postal code |
78352 |
| Country |
France |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE17402 |
Genome-wide comparison of EpiSC lines derived from fertilized (FT) and somatic cell nuclear transfer (NT) embryos |
|
