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Sample GSM434929 Query DataSets for GSM434929
Status Public on Dec 08, 2011
Title Mouse 583 proximal colon
Sample type RNA
 
Source name Healthy male, autoclaved chow diet
Organism Mus musculus
Characteristics tissue: proximal colon
strain: CONV-R
genotype: Myd88 -/-
Extracted molecule total RNA
Extraction protocol RNA was isolated using the RNeasy mini kit or the RNeasy Lipid tissue mini kit (QIAGEN), according to the manufacturer's instructions.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Samples were hybridized with GeneChip Mouse Gene 1.0 ST Arrays (Affymetrix) and scanned at the Uppsala Array Platform microarray core facility (Sweden).
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description RMA expression value derived from Expression Console software; gene level analysis
Data processing Raw data were processed with the Expression Console software (Affymetrix) for background correction and normalization. Downstream data analysis and statistical evaluations were performed using customized matlab scripts.
 
Submission date Jul 30, 2009
Last update date Dec 08, 2011
Contact name Erik Larsson
E-mail(s) erik.larsson@wlab.gu.se
Phone 0046707992946
Organization name University of Gothenburg
Department Wallenberg Laboratory
Street address Bruna stråket 16
City Gothenburg
ZIP/Postal code SE 413 45
Country Sweden
 
Platform ID GPL6246
Series (1)
GSE17438 Transcriptional profiling of 77 tissue samples from germ-free and conventionally raised mice.

Data table header descriptions
ID_REF
VALUE RMA expression value

Data table
ID_REF VALUE
10338001 12.6132
10338002 7.18842
10338003 10.9623
10338004 9.97182
10338005 2.54415
10338006 2.83412
10338007 3.24177
10338008 4.26108
10338009 9.44292
10338010 2.6157
10338011 6.45857
10338012 2.70562
10338013 2.47815
10338014 2.56013
10338015 2.50552
10338016 8.57134
10338017 13.6023
10338018 7.72028
10338019 5.88653
10338020 9.41073

Total number of rows: 35557

Table truncated, full table size 586 Kbytes.




Supplementary file Size Download File type/resource
GSM434929.CEL.gz 4.0 Mb (ftp)(http) CEL
GSM434929.chp.gz 270.3 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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