|
| Status |
Public on Dec 08, 2011 |
| Title |
Mouse 599 proximal colon |
| Sample type |
RNA |
| |
|
| Source name |
Healthy male, autoclaved chow diet
|
| Organism |
Mus musculus |
| Characteristics |
tissue: proximal colon
strain: GF
genotype: WT
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the RNeasy mini kit or the RNeasy Lipid tissue mini kit (QIAGEN), according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Mouse Gene 1.0 ST Arrays (Affymetrix) and scanned at the Uppsala Array Platform microarray core facility (Sweden).
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
RMA expression value derived from Expression Console software; gene level analysis
|
| Data processing |
Raw data were processed with the Expression Console software (Affymetrix) for background correction and normalization. Downstream data analysis and statistical evaluations were performed using customized matlab scripts.
|
| |
|
| Submission date |
Jul 30, 2009 |
| Last update date |
Dec 08, 2011 |
| Contact name |
Erik Larsson |
| E-mail(s) |
erik.larsson@wlab.gu.se
|
| Phone |
0046707992946
|
| Organization name |
University of Gothenburg
|
| Department |
Wallenberg Laboratory
|
| Street address |
Bruna stråket 16
|
| City |
Gothenburg |
| ZIP/Postal code |
SE 413 45 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE17438 |
Transcriptional profiling of 77 tissue samples from germ-free and conventionally raised mice. |
|
