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Sample GSM435175 Query DataSets for GSM435175
Status Public on Aug 17, 2010
Title Total RNA purified from MEFs derived from a WT mouse rep 2
Sample type RNA
 
Source name A5_rep2
Organism Mus musculus
Characteristics genotype: WT
Growth protocol MEFs were isolated from 14.5d pregnant females as previously described (Le Bacquer et al. 2007 The Journal of Clinical Investigation). WT and KI MEFs (5 x106) immortalized by passage were grown in DMEM supplemented with 10% FBS.
Extracted molecule total RNA
Extraction protocol Polysomal and total cytoplasmic RNA was isolated as described (Larsson et al. 2007, Cancer Research). Fractions containing mRNAs bound to >3 ribosomes were pooled and designated heavy polysomal RNA. RNA isolation procedure was performed in two independent experiments from one pair of KI and WT MEFs.
Label biotin
Label protocol 250ng RNA (polysomal or total) was labelled using the single round Illumina protocol
 
Hybridization protocol Standard hybridization optimized by Illumina
Scan protocol Standard scan protocoll optimized by Illumina
Description none
Data processing Data was transformed using Variance Stabilizing Transformation (VST) and normalized using Robust Spline Normalization (RSN) in the statistical environment R using the lumi package.
 
Submission date Jul 31, 2009
Last update date Aug 17, 2010
Contact name Ola Larsson
E-mail(s) ola.larsson@ki.se
Phone +46 (0)8 517 73280
Organization name Karolinska Institutet
Department Department of oncology-pathology
Street address CCK, R8:01
City Stockholm
ZIP/Postal code 171 76
Country Sweden
 
Platform ID GPL6885
Series (1)
GSE17451 eIF4E phosphorylation promotes tumorigenesis and is associated with prostate cancer progression

Data table header descriptions
ID_REF
VALUE Normalized transformed log2 expression level

Data table
ID_REF VALUE
ILMN_2896528 11.19505365
ILMN_2721178 9.66824318
ILMN_3033922 9.33487419
ILMN_3092673 10.68278058
ILMN_2816356 7.66111391
ILMN_2808939 11.15736506
ILMN_2634564 11.70434705
ILMN_2737647 7.67647948
ILMN_2734484 8.20972442
ILMN_2952292 8.76567705
ILMN_2699078 7.65559059
ILMN_1213681 9.19519035
ILMN_2735413 7.66164297
ILMN_2735415 7.68873495
ILMN_2891688 10.04650545
ILMN_2637698 8.37275812
ILMN_2674228 10.21379628
ILMN_2601546 7.71493149
ILMN_1230831 7.62154920
ILMN_2848071 7.95616392

Total number of rows: 25697

Table truncated, full table size 604 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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