|
| Status |
Public on Aug 17, 2010 |
| Title |
Polysomal RNA purified from MEFs derived from a WT mouse rep 2 |
| Sample type |
other |
| |
|
| Source name |
A5_rep2
|
| Organism |
Mus musculus |
| Characteristics |
genotype: WT
|
| Growth protocol |
MEFs were isolated from 14.5d pregnant females as previously described (Le Bacquer et al. 2007 The Journal of Clinical Investigation). WT and KI MEFs (5 x106) immortalized by passage were grown in DMEM supplemented with 10% FBS.
|
| Extracted molecule |
other |
| Extraction protocol |
Polysomal and total cytoplasmic RNA was isolated as described (Larsson et al. 2007, Cancer Research). Fractions containing mRNAs bound to >3 ribosomes were pooled and designated heavy polysomal RNA. RNA isolation procedure was performed in two independent experiments from one pair of KI and WT MEFs.
|
| Label |
biotin
|
| Label protocol |
250ng RNA (polysomal or total) was labelled using the single round Illumina protocol
|
| |
|
| Hybridization protocol |
Standard hybridization optimized by Illumina
|
| Scan protocol |
Standard scan protocoll optimized by Illumina
|
| Description |
none
|
| Data processing |
Data was transformed using Variance Stabilizing Transformation (VST) and normalized using Robust Spline Normalization (RSN) in the statistical environment R using the lumi package.
|
| |
|
| Submission date |
Jul 31, 2009 |
| Last update date |
Aug 17, 2010 |
| Contact name |
Ola Larsson |
| E-mail(s) |
ola.larsson@ki.se
|
| Phone |
+46 (0)8 517 73280
|
| Organization name |
Karolinska Institutet
|
| Department |
Department of oncology-pathology
|
| Street address |
CCK, R8:01
|
| City |
Stockholm |
| ZIP/Postal code |
171 76 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE17451 |
eIF4E phosphorylation promotes tumorigenesis and is associated with prostate cancer progression |
|
