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Sample GSM435178 Query DataSets for GSM435178
Status Public on Aug 17, 2010
Title Total RNA purified from MEFs derived from a EIF4E-KI mouse rep 2
Sample type RNA
 
Source name A4_rep2
Organism Mus musculus
Characteristics genotype: EIF4E-KI
Growth protocol MEFs were isolated from 14.5d pregnant females as previously described (Le Bacquer et al. 2007 The Journal of Clinical Investigation). WT and KI MEFs (5 x106) immortalized by passage were grown in DMEM supplemented with 10% FBS.
Extracted molecule total RNA
Extraction protocol Polysomal and total cytoplasmic RNA was isolated as described (Larsson et al. 2007, Cancer Research). Fractions containing mRNAs bound to >3 ribosomes were pooled and designated heavy polysomal RNA. RNA isolation procedure was performed in two independent experiments from one pair of KI and WT MEFs.
Label biotin
Label protocol 250ng RNA (polysomal or total) was labelled using the single round Illumina protocol
 
Hybridization protocol Standard hybridization optimized by Illumina
Scan protocol Standard scan protocoll optimized by Illumina
Description none
Data processing Data was transformed using Variance Stabilizing Transformation (VST) and normalized using Robust Spline Normalization (RSN) in the statistical environment R using the lumi package.
 
Submission date Jul 31, 2009
Last update date Aug 17, 2010
Contact name Ola Larsson
E-mail(s) ola.larsson@ki.se
Phone +46 (0)8 517 73280
Organization name Karolinska Institutet
Department Department of oncology-pathology
Street address CCK, R8:01
City Stockholm
ZIP/Postal code 171 76
Country Sweden
 
Platform ID GPL6885
Series (1)
GSE17451 eIF4E phosphorylation promotes tumorigenesis and is associated with prostate cancer progression

Data table header descriptions
ID_REF
VALUE Normalized transformed log2 expression level

Data table
ID_REF VALUE
ILMN_2896528 11.21165819
ILMN_2721178 9.64899234
ILMN_3033922 9.56207866
ILMN_3092673 10.99402706
ILMN_2816356 7.69599355
ILMN_2808939 10.78121747
ILMN_2634564 11.23120931
ILMN_2737647 7.68808760
ILMN_2734484 7.93099849
ILMN_2952292 8.45381552
ILMN_2699078 7.65449217
ILMN_1213681 9.41136108
ILMN_2735413 7.67072106
ILMN_2735415 7.65271396
ILMN_2891688 10.36816510
ILMN_2637698 8.59884801
ILMN_2674228 10.33505090
ILMN_2601546 7.77581139
ILMN_1230831 7.67512101
ILMN_2848071 7.88396162

Total number of rows: 25697

Table truncated, full table size 604 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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