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| Status |
Public on Oct 14, 2020 |
| Title |
miR-210 mutant 3 |
| Sample type |
SRA |
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| Source name |
adult fly heads
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| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: miR-210 mutant
tissue: adult heads
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extractions was performed using Trizol.
Libraries were prepared according to the Illumina protocol and sequenced on an Illumina novaseq6000 platform
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Description |
M22-3
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| Data processing |
Basic reads quality check was performed with FastQC
Reads were mapped to the Drosophila melanogaster reference genome assembly (version dm6.09, 2016) using Hisat2 and gene quantification was carried out using FeatureCounts.
Differential expression analysis was performed using DEseq.
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| Submission date |
Mar 04, 2020 |
| Last update date |
Oct 14, 2020 |
| Contact name |
Wanzhong Ge |
| E-mail(s) |
wanzhongge@zju.edu.cn
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| Phone |
86-0571-88981726
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| Organization name |
Zhejiang University
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| Street address |
Yuhangtang Road 866
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| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310058 |
| Country |
China |
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| Platform ID |
GPL25244 |
| Series (1) |
| GSE146335 |
Transcriptome analysis of w1118 and miR-210 mutant adult fly heads |
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| Relations |
| BioSample |
SAMN14278869 |
| SRA |
SRX7846765 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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