|
| Status |
Public on Mar 01, 2010 |
| Title |
MZ at 2,5 hpf replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
MZ whole embryo
|
| Organism |
Danio rerio |
| Characteristics |
hours post fertilization: 2.5
stage: 256 cell
|
| Treatment protocol |
Embryos of the genotypes indicated were precisely staged either following in vitro fertilization (t=0 hpf), or using natural crosses and staging at the 4-cell stage (t=1 hpf)
|
| Growth protocol |
WT embryos of AB x TÜB strain crosses (www.ZFIN.org) and MZ embryos carrying the m793 allele of the spg mutation were used
|
| Extracted molecule |
total RNA |
| Extraction protocol |
60-100 embryos per sample were snap-frozen in liquid nitrogen, and total RNA was isolated using the RNA Easy kit (Qiagen). Sample quality was assessed in an Agilent Bioanalyzer 2100, using the RNA 6000 nano Assay Kit
|
| Label |
Cy5
|
| Label protocol |
Samples were processed by Agilent Technologies Two-Color Microarray-Based Gene Expression Analysis kit according to Agilent Two-color Microarray based gene expression analysis protocol
|
| |
|
| Hybridization protocol |
Agilent Gene Expression Hybridization Kit was used . Micorarrays were hybridized in the Agilent 2545A hybridization oven according Agilent Two-color Microarray based gene expression analysis protocol
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner
|
| Description |
MZ to WT comparison 256 cell Replicate 3
|
| Data processing |
Data were extracted using Agilent Feature Extraction Software and GE2-v5_95_Feb07 protocoll.
|
| |
|
| Submission date |
Aug 14, 2009 |
| Last update date |
Feb 06, 2023 |
| Contact name |
Daria Onichtchouk |
| E-mail(s) |
daria.onichtchouk@biologie.uni-freiburg.de
|
| Phone |
00497612032595
|
| Organization name |
University of Freiburg
|
| Street address |
Hauptstrasse 1
|
| City |
Freiburg |
| ZIP/Postal code |
79104 |
| Country |
Germany |
| |
|
| Platform ID |
GPL7302 |
| Series (2) |
| GSE17655 |
Experiment 1: Developmental time curve in wild-type and MZspg embryos |
| GSE17667 |
Pou5f1 transcription targets in zebrafish |
|
