|
| Status |
Public on Mar 01, 2010 |
| Title |
MZspg 30% epiboly stage replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
MZspg whole embryo
|
| Organism |
Danio rerio |
| Characteristics |
hours post fertilization: 4,7 hpf
stage: 30% epiboly
|
| Treatment protocol |
Embryos of the genotypes indicated were precisely staged either following in vitro fertilization (t=0 hpf), or using natural crosses and staging at the 4-cell stage (t=1 hpf)
|
| Growth protocol |
WT embryos of AB x TÜB strain crosses (www.ZFIN.org) and MZ embryos carrying the m793 allele of the spg mutation were used
|
| Extracted molecule |
total RNA |
| Extraction protocol |
120 embryos per sample were snap-frozen in liquid nitrogen, and total RNA was isolated using the RNA Easy kit (Qiagen). Sample quality was assessed in an Agilent Bioanalyzer 2100, using the RNA 6000 nano Assay Kit
|
| Label |
biotin
|
| Label protocol |
cRNA was amplified using the Ambion MessageAmp™ RNA Amplification Kit. Sample fragmentation, hybridization, washing and staining were performed according to Affymetrix Gene Chip Expression Analysis Technical Manual
|
| |
|
| Hybridization protocol |
Agilent Gene Expression Hybridization Kit was used . Micorarrays were hybridized in the Agilent 2545A hybridization oven according Agilent Two-color Microarray based gene expression analysis protocol
|
| Scan protocol |
Microarrays were scanned on a GeneChip scanner 3000.
|
| Description |
MZ of MZ to WT comparison 30% epiboly stage Replicate 2
|
| Data processing |
CEL files were loaded to the Refiner Array module of Genedata Expressionist software, submitted to quality inspection, classified and condensed using the MAS5 Affymetrix statistical algorithm.
|
| |
|
| Submission date |
Aug 14, 2009 |
| Last update date |
Feb 06, 2023 |
| Contact name |
Daria Onichtchouk |
| E-mail(s) |
daria.onichtchouk@biologie.uni-freiburg.de
|
| Phone |
00497612032595
|
| Organization name |
University of Freiburg
|
| Street address |
Hauptstrasse 1
|
| City |
Freiburg |
| ZIP/Postal code |
79104 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1319 |
| Series (2) |
| GSE17657 |
Experiment 4: Affymetrix validation array |
| GSE17667 |
Pou5f1 transcription targets in zebrafish |
|
