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Sample GSM4409032 Query DataSets for GSM4409032
Status Public on Jun 01, 2021
Title IgG: IgG MeDIP-seq
Sample type SRA
 
Source name Hypothalamus
Organism Mus musculus
Characteristics strain: C57/Bl6
tissue: Hypothalamus
antibody: BI-MECY
molecule subtype: Methylated cytosine
Extracted molecule genomic DNA
Extraction protocol Qiagen RNA/DNA extraction kit
100ng of DNA was used for library preparation using the Ion Xpress Plus Fragment Library Kit. Amplification was carried out for 8 cycles and purified using the Agnecourt AMPure XP PCR clean up kit.
 
Library strategy MeDIP-Seq
Library source genomic
Library selection 5-methylcytidine antibody
Instrument model Ion Torrent Proton
 
Data processing Bam files generated by IonTorrent Suite v5.2.0
Peaks were called using MACS2 v2.1
Differential peaks were identified using DiffBind
Peaks were annotated using Homer v4.8
Genome_build: mm11
 
Submission date Mar 12, 2020
Last update date Jun 01, 2021
Contact name Eamon Fitzgerald
E-mail(s) eamon.fitzgerald58@gmail.com
Organization name University of Edinburgh
Street address 47 Little France Cres
City Edinburgh
State/province Midlothian
ZIP/Postal code EH164TJ
Country United Kingdom
 
Platform ID GPL18635
Series (1)
GSE146892 DNA methylation immunoprecipitation of hypothalamic DNA following modified maternal separation (MMS)
Relations
BioSample SAMN14366879
SRA SRX7902315

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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