|
Status |
Public on Jun 01, 2021 |
Title |
IgG: IgG MeDIP-seq |
Sample type |
SRA |
|
|
Source name |
Hypothalamus
|
Organism |
Mus musculus |
Characteristics |
strain: C57/Bl6 tissue: Hypothalamus antibody: BI-MECY molecule subtype: Methylated cytosine
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Qiagen RNA/DNA extraction kit 100ng of DNA was used for library preparation using the Ion Xpress Plus Fragment Library Kit. Amplification was carried out for 8 cycles and purified using the Agnecourt AMPure XP PCR clean up kit.
|
|
|
Library strategy |
MeDIP-Seq |
Library source |
genomic |
Library selection |
5-methylcytidine antibody |
Instrument model |
Ion Torrent Proton |
|
|
Data processing |
Bam files generated by IonTorrent Suite v5.2.0 Peaks were called using MACS2 v2.1 Differential peaks were identified using DiffBind Peaks were annotated using Homer v4.8 Genome_build: mm11
|
|
|
Submission date |
Mar 12, 2020 |
Last update date |
Jun 01, 2021 |
Contact name |
Eamon Fitzgerald |
E-mail(s) |
eamon.fitzgerald58@gmail.com
|
Organization name |
University of Edinburgh
|
Street address |
47 Little France Cres
|
City |
Edinburgh |
State/province |
Midlothian |
ZIP/Postal code |
EH164TJ |
Country |
United Kingdom |
|
|
Platform ID |
GPL18635 |
Series (1) |
GSE146892 |
DNA methylation immunoprecipitation of hypothalamic DNA following modified maternal separation (MMS) |
|
Relations |
BioSample |
SAMN14366879 |
SRA |
SRX7902315 |