|
Status |
Public on May 09, 2011 |
Title |
hi559 Wild Type Repl3 |
Sample type |
RNA |
|
|
Source name |
Phenotyped whole wild type embryo at 5 dpf
|
Organism |
Danio rerio |
Characteristics |
strain: cdipt^hi559Tg phenotype: Wild Type age: 5 dpf replicate: 3
|
Treatment protocol |
Embryos were sorted based on phenotype into mutant and wild type embryos.
|
Growth protocol |
Embryos from an incross of heterozygous cdipt^hi559Tg/+ parents were grown until 5 dpf in standard embryo media at 28° C.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was prepared from 25 embryos for each phenotype with RNeasy (QIAGEN).
|
Label |
biotin
|
Label protocol |
Affymetrix GeneChip One-Cycle Target Labeling and Control Reagents, manufacturer's protocol.
|
|
|
Hybridization protocol |
Hybridization protocol used was EukGE-WS2v4 on an Affymetrix GeneChip Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned with an Affymetrix scanner according to the manufacturer's protocol.
|
Description |
WT3
|
Data processing |
Expression values were extracted with RMA (Affy Library in R). Each array was normalized by log2-transformation, subtraction of median, and division with standard deviation.
|
|
|
Submission date |
Aug 18, 2009 |
Last update date |
May 09, 2011 |
Contact name |
Carsten Stuckenholz |
E-mail(s) |
cstucken@pitt.edu
|
Phone |
(412) 648-3159
|
Fax |
(412) 383-5918
|
Organization name |
University of Pittsburgh
|
Department |
Department of Medicine, Division of Hematology and Oncology
|
Lab |
Bahary
|
Street address |
3501 Fifth Avenue, Room 5065.5B
|
City |
Pittsburgh |
State/province |
PA |
ZIP/Postal code |
15260 |
Country |
USA |
|
|
Platform ID |
GPL1319 |
Series (1) |
GSE17711 |
Lack of de novo phosphatidylinositol synthesis leads to endoplasmic reticulum stress and hepatic steatosis in cdipt-deficient zebrafish |
|