|
| Status |
Public on Aug 28, 2009 |
| Title |
Very large tumor 3557U |
| Sample type |
RNA |
| |
|
| Source name |
Size: very large, tumor
|
| Organism |
Mus musculus |
| Characteristics |
size: very large
tissue: neuroblastoma tumor
tumor weight: 1.65 g
life span: 45 days
|
| Growth protocol |
TH-MYCN transgenic mice were bred to homozygosity and examined with high frequency ultrasonography (Vevo770 Visual Sonics Inc) three times per week was initiated immediately upon weaning at day 28 to detect asymptomatic tumors and track tumor growth. Mice were sacrificed when tumor dimensions were within one of four predetermined size ranges.Six replicates harvested within four size cohorts.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from 10µm frozen sections using Qiagen RNeasy Micro Kit (Qiagen, Valencia, CA). Tumor total RNA (76ng-1.6µg) was used to generate cDNA targets.
|
| Label |
Digoxigenin
|
| Label protocol |
standard ABI protocol
|
| |
|
| Hybridization protocol |
Samples hybridized using Applied Biosystems Mouse Genome Survey Microarray version 1.0 standard protocol
|
| Scan protocol |
ABI Mouse Genome Survery Microarray version 1.0 standard scanning protocol
|
| Description |
n/a
|
| Data processing |
ABI Mouse Genome Survery Microarray version 1.0 standard data processing protocol. Raw probe-level measurements were processed by quantile normalization for sample quality control.
|
| |
|
| Submission date |
Aug 20, 2009 |
| Last update date |
Aug 27, 2009 |
| Contact name |
Jayanti Jagannathan |
| E-mail(s) |
jagannathan@email.chop.edu
|
| Phone |
2674262286
|
| Organization name |
Childrens Hospital of Philadelphia
|
| Department |
Oncology
|
| Street address |
34th & Civic center blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL2995 |
| Series (1) |
| GSE17740 |
Serial transcriptome analysis and cross species integration identifies CENPE as novel neuroblastoma target |
|
