|
| Status |
Public on Jun 01, 2021 |
| Title |
MMS 5 |
| Sample type |
SRA |
| |
|
| Source name |
Hypothalamus tissue
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/Bl6
group: MMS
|
| Treatment protocol |
RNA was extraction from the P6 hypothalamus after
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNA/DNA extraction kit from Qiagen using standard protocols
Library preparation was done using the QuantSeq 3’ RNA-Seq Library Prep Kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent Proton |
| |
|
| Data processing |
Raw pH DAT files were produced directly from the Ion platform, which were then converted to flow signals and aligned to the mm10 reference genome in an automated workflow
Further downstream analysis was done using Galaxy and differential gene expression done with Degust software.
Genome_build: mm10
|
| |
|
| Submission date |
Mar 23, 2020 |
| Last update date |
Jun 01, 2021 |
| Contact name |
Eamon Fitzgerald |
| E-mail(s) |
eamon.fitzgerald58@gmail.com
|
| Organization name |
University of Edinburgh
|
| Street address |
47 Little France Cres
|
| City |
Edinburgh |
| State/province |
Midlothian |
| ZIP/Postal code |
EH164TJ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL18635 |
| Series (1) |
| GSE147375 |
The assessment of differential expression in the hypothalamus following MMS |
|
| Relations |
| BioSample |
SAMN14430386 |
| SRA |
SRX7973548 |
