GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM44330 Query DataSets for GSM44330
Status Public on Mar 02, 2005
Title Brown Fat, Male (MoBF.Male)
Sample type MPSS
Source name Brown Fat, Male total RNA
Organism Mus musculus
Extracted molecule total RNA
Description Strain: C57BL6
Brown Fat, Male total RNA (MoBF.Male) received from National Human Genome Research Institute was analyzed through Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the previous publications (Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4): 1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634). Briefly, the mRNA was reverse transcribed and the cDNA was digested with Dpn II. The 20 bases adjacent to the 3? most Dpn II site was cloned into a Megaclone vector. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples.
Library MoBF.Male.sig21
Cell type Brown Fat, Male
Source NHGRI
RNA isolation LYNX mRNA QC passed
cDNA library:
Library DpnII restriction - (signature cloning using MmeI)
Sequence length 20 bp
MoBF.Male_sig21.5209F.a-20 10/19/2004 364704 6310 QC Passed
MoBF.Male_sig21.5209W.a-20 10/18/2004 544420 6890 QC Passed
MoBF.Male_sig21.5209W.c-20 11/15/2004 621278 8371 QC Passed
MoBF.Male_sig21.5364F.a-20 1/26/2005 509470 8766 QC Passed
MoBF.Male_sig21.5364F.b-20 2/18/2005 402025 6076 QC Passed
Run group:
Total Beads successfully sequenced - 2441897
Processed Signatures - 12633
Submission date Mar 02, 2005
Last update date Dec 14, 2005
Contact name Daixing Zhou
Phone 510-670-9441
Fax 510-670-9302
Organization name Solexa
Department Bioinformatics
Street address 25861 Industrial Blvd
City Hayward
State/province CA
ZIP/Postal code 94545
Country USA
Platform ID GPL1010
Series (1)
GSE1581 MPSS mouse transcriptome analysis project

Data table header descriptions
ID_REF Signature - 20 nt sequence including DpnII site (GATC)
VALUE Mean abundance for a signature derived from all MPSS runs for the sample, in transcripts per million (TPM)
ST_DEV Standard deviation of the mean abundance from multiple MPSS runs
REP Replication_global

Total number of rows: 7889

Table truncated, full table size 234 Kbytes.

Supplementary file Size Download File type/resource
GSM44330.raw.txt.gz 164.7 Kb (ftp)(http) TXT

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap