|
| Status |
Public on Jun 17, 2010 |
| Title |
retinal plus lens-treated tissue rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Amacrine cell layer from plus lens-treated bird
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: Amacrine cell layer
|
| Treatment protocol |
Chicks were monocularly treated with either -7D (n=3, growth stimulant) or +7D (n=3, growth suppressant) lenses for 24hrs (12pm to 12pm), with untreated age-matched chicks serving as a control (n=3). Chicks were kept under a 12:12hr light-dark cycle (lights on 7am, lights off 7pm, with an illumination of 500lux).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from the amacrine cell layer was isolated from laser-capture microdissected (Zeiss, Bernried, Germany) 10µm thick sections using a RNeasy Micro Kit (Qiagen, Hilden, Germany), following the manufactures instructions. Labeled cRNA was prepared from three separate samples per condition and hybridized to Affymetrix GeneChip Chicken Genome arrays.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10-100ng of total RNA (see manufacturer´s website: Expression Analysis Technical Manual, Two-Cycle Target Labeling Assay, Affymetrix).
|
| |
|
| Hybridization protocol |
see manufacturer's website (http://www.affymetrix.com/)
|
| Scan protocol |
see manufacturer's website (http://www.affymetrix.com/)
|
| Description |
retinal plus lens-treated tissue rep2
|
| Data processing |
GC-RMA
|
| |
|
| Submission date |
Aug 21, 2009 |
| Last update date |
Aug 21, 2009 |
| Contact name |
Regan Ashby |
| E-mail(s) |
regan.ashby@klinikum.uni-tuebingen.de
|
| Organization name |
University of Tuebingen
|
| Department |
Section of Neurobiology of the Eye
|
| Street address |
Calwerstrasse 7/1
|
| City |
Tuebingen |
| ZIP/Postal code |
72076 |
| Country |
Germany |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE17758 |
Microarray analysis of changes in gene expression within the amacrine cell layer of chicks following optical defocus |
|
