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Sample GSM443532 Query DataSets for GSM443532
Status Public on Jun 17, 2010
Title retinal minus lens-treated tissue rep1
Sample type RNA
 
Source name Amacrine cell layer from plus minus-treated bird
Organism Gallus gallus
Characteristics tissue: Amacrine cell layer
Treatment protocol Chicks were monocularly treated with either -7D (n=3, growth stimulant) or +7D (n=3, growth suppressant) lenses for 24hrs (12pm to 12pm), with untreated age-matched chicks serving as a control (n=3). Chicks were kept under a 12:12hr light-dark cycle (lights on 7am, lights off 7pm, with an illumination of 500lux).
Extracted molecule total RNA
Extraction protocol Total RNA from the amacrine cell layer was isolated from laser-capture microdissected (Zeiss, Bernried, Germany) 10µm thick sections using a RNeasy Micro Kit (Qiagen, Hilden, Germany), following the manufactures instructions. Labeled cRNA was prepared from three separate samples per condition and hybridized to Affymetrix GeneChip Chicken Genome arrays.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10-100ng of total RNA (see manufacturer´s website: Expression Analysis Technical Manual, Two-Cycle Target Labeling Assay, Affymetrix).
 
Hybridization protocol see manufacturer's website (http://www.affymetrix.com/)
Scan protocol see manufacturer's website (http://www.affymetrix.com/)
Description retinal minus lens-treated tissue rep1
Data processing GC-RMA
 
Submission date Aug 21, 2009
Last update date Aug 21, 2009
Contact name Regan Ashby
E-mail(s) regan.ashby@klinikum.uni-tuebingen.de
Organization name University of Tuebingen
Department Section of Neurobiology of the Eye
Street address Calwerstrasse 7/1
City Tuebingen
ZIP/Postal code 72076
Country Germany
 
Platform ID GPL3213
Series (1)
GSE17758 Microarray analysis of changes in gene expression within the amacrine cell layer of chicks following optical defocus

Data table header descriptions
ID_REF
VALUE GC-RMA based signal intensity

Data table
ID_REF VALUE
AB112960.CDS1.S1_at 8.197248
AF004856.CDS1.S1_s_at 4.8879633
AF004857.CDS1.S1_s_at 5.696453
AF004857.CDS2.S1_s_at 4.1318946
AF004857.CDS3.S1_s_at 5.4208517
AF059716.CDS1.S1_at 8.355549
AF059717.CDS1.S1_at 7.2595634
AF059721.CDS1.S1_at 4.3482165
AF059724.CDS1.S1_at 5.264841
AF059725.CDS1.S1_at 4.169791
AF176590.CDS1.S1_s_at 5.3823876
AF176591.CDS1.S1_s_at 5.1763496
AF176592.CDS1.S1_s_at 10.493125
AF176592.CDS2.S1_s_at 12.370428
AF218359.CDS1.S1_at 5.1977525
AF218359.CDS2.S1_at 3.7913966
AF218359.CDS3.S1_at 4.986759
AF243438.CDS1.S1_s_at 4.6095386
AF243438.CDS10.S1_s_at 7.2604766
AF243438.CDS11.S1_s_at 4.730125

Total number of rows: 38535

Table truncated, full table size 1101 Kbytes.




Supplementary file Size Download File type/resource
GSM443532.CEL.gz 3.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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