|
| Status |
Public on Apr 07, 2020 |
| Title |
WT-CR-1 |
| Sample type |
RNA |
| |
|
| Source name |
liver, normal-fat diet and 30~35% caloric restriction as in AL (CR)
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
start age: 2 months
treatment time: 3 months
Sex: Male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from 20-30 mg tissue using TRIzol reagent (Invitrogen Life Technologies, USA) and RNasey Mini Kit (Qiagen, Germany) following the manufacturer's instructions. Yield and purity of RNA was determined by NanoDrop ND-1000 spectrophotometer (Nanodrop technologies, USA).
|
| Label |
Hy3
|
| Label protocol |
The samples are labeled using the miRCURYTM Array Power Labeling kit (Cat #208032-A, Exiqon) as instruction
|
| |
|
| Hybridization protocol |
The samples were hybridized on the miRCURY™ LNA Array (v.11.0) with kit (Cat #208021, Exiqon) as instruction.
|
| Scan protocol |
Scanning is performed with the Axon GenePix 4000B microarray scanner. GenePix pro V6.0 is used to read the raw intensity of the image.
|
| Data processing |
The intensity of green signal is calculated after background subtraction and replicated spots on the same slide have been averaged by getting a median intensity. We use Median Normalization Method to obtain "Normalized Data", Normalized Data=(Foreground-Background)/median, the median is 50 percent quantile of microRNA intensity which is larger than 50 in all samples after background correction.
|
| |
|
| Submission date |
Apr 06, 2020 |
| Last update date |
Apr 07, 2020 |
| Contact name |
Huan Gong |
| E-mail(s) |
gonghuan3861@bjhmoh.cn
|
| Phone |
86-10-58115045
|
| Organization name |
Beijing Institute of Geriatrics, Beijing Hospital, National Center of Gerontology
|
| Street address |
No.1 Dahua Road, Dong Dan,
|
| City |
Beijing |
| ZIP/Postal code |
100730 |
| Country |
China |
| |
|
| Platform ID |
GPL17107 |
| Series (1) |
| GSE148148 |
microRNAs array of livers from three different lifestyle modification mice models |
|
