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Status |
Public on Apr 07, 2020 |
Title |
WT-EX-1 |
Sample type |
RNA |
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Source name |
liver, ad libitum, normal-fat diet with exercise(EX)
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Organism |
Mus musculus |
Characteristics |
tissue: liver start age: 2 months treatment time: 3 months Sex: Male
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from 20-30 mg tissue using TRIzol reagent (Invitrogen Life Technologies, USA) and RNasey Mini Kit (Qiagen, Germany) following the manufacturer's instructions. Yield and purity of RNA was determined by NanoDrop ND-1000 spectrophotometer (Nanodrop technologies, USA).
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Label |
Hy3
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Label protocol |
The samples are labeled using the miRCURYTM Array Power Labeling kit (Cat #208032-A, Exiqon) as instruction
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Hybridization protocol |
The samples were hybridized on the miRCURY™ LNA Array (v.11.0) with kit (Cat #208021, Exiqon) as instruction.
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Scan protocol |
Scanning is performed with the Axon GenePix 4000B microarray scanner. GenePix pro V6.0 is used to read the raw intensity of the image.
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Data processing |
The intensity of green signal is calculated after background subtraction and replicated spots on the same slide have been averaged by getting a median intensity. We use Median Normalization Method to obtain "Normalized Data", Normalized Data=(Foreground-Background)/median, the median is 50 percent quantile of microRNA intensity which is larger than 50 in all samples after background correction.
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Submission date |
Apr 06, 2020 |
Last update date |
Apr 07, 2020 |
Contact name |
Huan Gong |
E-mail(s) |
gonghuan3861@bjhmoh.cn
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Phone |
86-10-58115045
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Organization name |
Beijing Institute of Geriatrics, Beijing Hospital, National Center of Gerontology
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Street address |
No.1 Dahua Road, Dong Dan,
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City |
Beijing |
ZIP/Postal code |
100730 |
Country |
China |
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Platform ID |
GPL17107 |
Series (1) |
GSE148148 |
microRNAs array of livers from three different lifestyle modification mice models |
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