|
| Status |
Public on Aug 28, 2009 |
| Title |
Day 0 ganglia D0_R0581_V2 |
| Sample type |
RNA |
| |
|
| Source name |
Day 0, ganglia
|
| Organism |
Mus musculus |
| Characteristics |
time point: day 0
tissue: ganglia
|
| Growth protocol |
TH-MYCN transgenic mice were bred to homozygosity and sympathetic ganglia containing foci of neuroblast hyperplasia were harvested at Day 0, Day 7 and Day 14 of life. Two tumors were harvested after weaning at Day 28 when tumor dimensions were in the Small tumor predetermined size range
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from 10µm frozen sections using Qiagen RNeasy Micro Kit (Qiagen, Valencia, CA). Tumor total RNA (76ng-1.6µg) was used to generate cDNA targets.
|
| Label |
Digoxigenin
|
| Label protocol |
standard ABI protocol
|
| |
|
| Hybridization protocol |
Samples hybridized using Applied Biosystems Mouse Genome Survey Microarray version 1.0 standard protocol
|
| Scan protocol |
ABI Mouse Genome Survery Microarray version 1.0 standard scanning protocol
|
| Description |
n/a
|
| Data processing |
ABI Mouse Genome Survery Microarray version 1.0 standard data processing protocol. Raw probe-level measurements were processed by quantile normalization for sample quality control.
|
| |
|
| Submission date |
Aug 27, 2009 |
| Last update date |
Aug 27, 2009 |
| Contact name |
Jayanti Jagannathan |
| E-mail(s) |
jagannathan@email.chop.edu
|
| Phone |
2674262286
|
| Organization name |
Childrens Hospital of Philadelphia
|
| Department |
Oncology
|
| Street address |
34th & Civic center blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL2995 |
| Series (1) |
| GSE17740 |
Serial transcriptome analysis and cross species integration identifies CENPE as novel neuroblastoma target |
|
