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Sample GSM446413 Query DataSets for GSM446413
Status Public on Oct 30, 2009
Title Hemocytes GFP-CON, biological rep2
Sample type RNA
 
Source name hemocytes isolated from Anopheles gambiae female adults 24-28h after P. berghei infection
Organism Anopheles gambiae
Characteristics infection status: P. berghei infection
mean number of oocysts: 60
oocyst prevalence: 79
cell type: hemocyte
Treatment protocol Hemocytes were harvested from 500 infected females by proboscis clipping and slight thorax-squeezing with dissection forceps. A hemolymph drop from each mosquito was collected directly into RNeasy’s buffer RLT (Qiagen) and stored at -80ºC
Extracted molecule total RNA
Extraction protocol Total RNA was extracted according to the RNeasy Mini protocol for animal cells (Qiagen)
Label biotin
Label protocol Total RNA was biotinylated according to the Two-Cycle Eukaryotic Target Labeling protocol (Affymetrix) using 80 ng of total RNA as a starting amount
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Plasmodium/Anopheles Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description gene expression data from hemocytes isolated from Anopheles gambiae female adults 24-28h after P. berghei infection
Data processing Analysis of the microarrays was performed using the GCOS 1.4 (Affymetrix).
 
Submission date Aug 28, 2009
Last update date Sep 30, 2009
Contact name Kristin Michel
E-mail(s) kmichel@ksu.edu
Organization name Kansas State University
Department Biology
Street address 267 Chalmers Hall
City Manhattan
State/province KS
ZIP/Postal code 66506
Country USA
 
Platform ID GPL1321
Series (1)
GSE17866 Expression profiling of hemocytes from Anopheles gambiae after malaria parasite infection

Data table header descriptions
ID_REF
VALUE GCOS 1.4 MAS 5 signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1009.54 P 0.000753643
AFFX-BioB-M_at 2226.46 P 9.4506e-05
AFFX-BioB-3_at 1485.91 P 5.16732e-05
AFFX-BioC-5_at 5044.35 P 5.16732e-05
AFFX-BioC-3_at 2938.07 P 5.16732e-05
AFFX-BioDn-5_at 11747.4 P 4.42873e-05
AFFX-BioDn-3_at 23527.6 P 8.14279e-05
AFFX-CreX-5_at 53708.4 P 4.42873e-05
AFFX-CreX-3_at 101376 P 4.42873e-05
AFFX-DapX-5_at 665.779 P 0.000340305
AFFX-DapX-M_at 4805.61 P 0.000146581
AFFX-DapX-3_at 19297.3 P 4.42873e-05
AFFX-LysX-5_at 31.9273 A 0.425962
AFFX-LysX-M_at 1031.3 P 0.00844047
AFFX-LysX-3_at 2970.39 P 4.42873e-05
AFFX-PheX-5_at 397.374 P 0.0113844
AFFX-PheX-M_at 1190.85 P 0.00110197
AFFX-PheX-3_at 2198.03 P 0.000126798
AFFX-ThrX-5_at 110.77 A 0.544587
AFFX-ThrX-M_at 677.451 P 0.0309764

Total number of rows: 22769

Table truncated, full table size 854 Kbytes.




Supplementary file Size Download File type/resource
GSM446413_Hemocyte_PbGFPCON_2.CEL.gz 1.7 Mb (ftp)(http) CEL
GSM446413_Hemocyte_PbGFPCON_2.CHP.gz 126.2 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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