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Sample GSM446417 Query DataSets for GSM446417
Status Public on Oct 30, 2009
Title Hemocytes CTRP-ko-EGFP, biological rep3
Sample type RNA
 
Source name hemocytes isolated from Anopheles gambiae female adults 24-28h after control treatment
Organism Anopheles gambiae
Characteristics infection status: non-infected
mean number of oocysts: 0
oocyst prevalence: 0
cell type: hemocyte
Treatment protocol Hemocytes were harvested from 500 infected females by proboscis clipping and slight thorax-squeezing with dissection forceps. A hemolymph drop from each mosquito was collected directly into RNeasy’s buffer RLT (Qiagen) and stored at -80ºC
Extracted molecule total RNA
Extraction protocol Total RNA was extracted according to the RNeasy Mini protocol for animal cells (Qiagen)
Label biotin
Label protocol Total RNA was biotinylated according to the Two-Cycle Eukaryotic Target Labeling protocol (Affymetrix) using 80 ng of total RNA as a starting amount
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Plasmodium/Anopheles Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description gene expression data from hemocytes isolated from Anopheles gambiae female adults 24-28h after control treatment
Data processing Analysis of the microarrays was performed using the GCOS 1.4 (Affymetrix).
 
Submission date Aug 28, 2009
Last update date Sep 30, 2009
Contact name Kristin Michel
E-mail(s) kmichel@ksu.edu
Organization name Kansas State University
Department Biology
Street address 267 Chalmers Hall
City Manhattan
State/province KS
ZIP/Postal code 66506
Country USA
 
Platform ID GPL1321
Series (1)
GSE17866 Expression profiling of hemocytes from Anopheles gambiae after malaria parasite infection

Data table header descriptions
ID_REF
VALUE GCOS 1.4 MAS 5 signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1186.57 P 0.00110197
AFFX-BioB-M_at 1688.48 P 0.0012475
AFFX-BioB-3_at 1433.13 P 8.14279e-05
AFFX-BioC-5_at 5109.18 P 5.16732e-05
AFFX-BioC-3_at 2885.58 P 5.16732e-05
AFFX-BioDn-5_at 11591.4 P 4.42873e-05
AFFX-BioDn-3_at 22985.1 P 0.000126798
AFFX-CreX-5_at 52285.5 P 4.42873e-05
AFFX-CreX-3_at 96585.5 P 4.42873e-05
AFFX-DapX-5_at 654.702 P 0.000581214
AFFX-DapX-M_at 4723.91 P 0.000581214
AFFX-DapX-3_at 15181.5 P 4.42873e-05
AFFX-LysX-5_at 173.049 P 0.0429619
AFFX-LysX-M_at 918.149 P 0.0309764
AFFX-LysX-3_at 2488.83 P 4.42873e-05
AFFX-PheX-5_at 396.953 P 0.0125468
AFFX-PheX-M_at 929.229 P 0.0032123
AFFX-PheX-3_at 2780.49 P 0.000340305
AFFX-ThrX-5_at 304.247 A 0.205732
AFFX-ThrX-M_at 559.1 M 0.0584438

Total number of rows: 22769

Table truncated, full table size 853 Kbytes.




Supplementary file Size Download File type/resource
GSM446417_Hemocyte_PbCTRPko_3.CEL.gz 1.8 Mb (ftp)(http) CEL
GSM446417_Hemocyte_PbCTRPko_3.CHP.gz 125.7 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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